A TILLING Platform for Functional Genomics in Brachypodium distachyon.
The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists...
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doaj-c7d504f5bf24457a86e70d3e1baf5e642020-11-25T01:41:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6550310.1371/journal.pone.0065503A TILLING Platform for Functional Genomics in Brachypodium distachyon.Marion DalmaisSébastien AntelmeSéverine Ho-Yue-KuangYin WangOlivier DarracqMadeleine Bouvier d'YvoireLaurent CézardFrédéric LégéeEddy BlondetNicolas OriaChristelle TroadecVéronique BrunaudLise JouaninHerman HöfteAbdelafid BendahmaneCatherine LapierreRichard SiboutThe new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists of DNA isolated from 5530 different families. Phenotypes were reported and organized in a phenotypic tree that is freely available online. The tilling platform was validated by the isolation of mutants for seven genes belonging to multigene families of the lignin biosynthesis pathway. In particular, a large allelic series for BdCOMT6, a caffeic acid O-methyl transferase was identified. Some mutants show lower lignin content when compared to wild-type plants as well as a typical decrease of syringyl units, a hallmark of COMT-deficient plants. The mutation rate was estimated at one mutation per 396 kb, or an average of 680 mutations per line. The collection was also used to assess the Genetically Effective Cell Number that was shown to be at least equal to 4 cells in Brachypodium distachyon. The mutant population and the TILLING platform should greatly facilitate functional genomics approaches in this model organism.http://europepmc.org/articles/PMC3686759?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marion Dalmais Sébastien Antelme Séverine Ho-Yue-Kuang Yin Wang Olivier Darracq Madeleine Bouvier d'Yvoire Laurent Cézard Frédéric Légée Eddy Blondet Nicolas Oria Christelle Troadec Véronique Brunaud Lise Jouanin Herman Höfte Abdelafid Bendahmane Catherine Lapierre Richard Sibout |
spellingShingle |
Marion Dalmais Sébastien Antelme Séverine Ho-Yue-Kuang Yin Wang Olivier Darracq Madeleine Bouvier d'Yvoire Laurent Cézard Frédéric Légée Eddy Blondet Nicolas Oria Christelle Troadec Véronique Brunaud Lise Jouanin Herman Höfte Abdelafid Bendahmane Catherine Lapierre Richard Sibout A TILLING Platform for Functional Genomics in Brachypodium distachyon. PLoS ONE |
author_facet |
Marion Dalmais Sébastien Antelme Séverine Ho-Yue-Kuang Yin Wang Olivier Darracq Madeleine Bouvier d'Yvoire Laurent Cézard Frédéric Légée Eddy Blondet Nicolas Oria Christelle Troadec Véronique Brunaud Lise Jouanin Herman Höfte Abdelafid Bendahmane Catherine Lapierre Richard Sibout |
author_sort |
Marion Dalmais |
title |
A TILLING Platform for Functional Genomics in Brachypodium distachyon. |
title_short |
A TILLING Platform for Functional Genomics in Brachypodium distachyon. |
title_full |
A TILLING Platform for Functional Genomics in Brachypodium distachyon. |
title_fullStr |
A TILLING Platform for Functional Genomics in Brachypodium distachyon. |
title_full_unstemmed |
A TILLING Platform for Functional Genomics in Brachypodium distachyon. |
title_sort |
tilling platform for functional genomics in brachypodium distachyon. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists of DNA isolated from 5530 different families. Phenotypes were reported and organized in a phenotypic tree that is freely available online. The tilling platform was validated by the isolation of mutants for seven genes belonging to multigene families of the lignin biosynthesis pathway. In particular, a large allelic series for BdCOMT6, a caffeic acid O-methyl transferase was identified. Some mutants show lower lignin content when compared to wild-type plants as well as a typical decrease of syringyl units, a hallmark of COMT-deficient plants. The mutation rate was estimated at one mutation per 396 kb, or an average of 680 mutations per line. The collection was also used to assess the Genetically Effective Cell Number that was shown to be at least equal to 4 cells in Brachypodium distachyon. The mutant population and the TILLING platform should greatly facilitate functional genomics approaches in this model organism. |
url |
http://europepmc.org/articles/PMC3686759?pdf=render |
work_keys_str_mv |
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