Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation
Long noncoding RNAs (lncRNAs) are emerging as key regulators of diverse cell functions and processes. However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. Here we used microarray profiling to determine lncRNA and mRNA expression in m...
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Online Access: | http://dx.doi.org/10.1155/2015/461038 |
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doaj-c7d7477b597d42059c88894e063c698e2020-11-24T20:55:12ZengHindawi LimitedInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/461038461038Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing RadiationJonathan Tang0Yurong Huang1David H. Nguyen2Sylvain V. Costes3Antoine M. Snijders4Jian-Hua Mao5Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USALong noncoding RNAs (lncRNAs) are emerging as key regulators of diverse cell functions and processes. However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. Here we used microarray profiling to determine lncRNA and mRNA expression in mammary glands of BALB/c and SPRET/EiJ mice after low-dose ionizing radiation (LDIR) exposure. We found that unirradiated mammary tissues of these strains differed significantly in baseline expressions of 290 lncRNAs. LDIR exposure (10 cGy) induced a significant change in the expression of many lncRNAs. The vast majority of lncRNAs identified to be differentially expressed after LDIR in either BALB/c or SPRET/EiJ had a significantly correlated expression pattern with at least one LDIR responsive mRNA. Functional analysis revealed that the response to LDIR in BALB/c mice is highly dynamic with enrichment for genes involved in tissue injury, inflammatory responses, and mammary gland development at 2, 4, and 8 weeks after LDIR, respectively. Our study demonstrates that genetic background strongly influences the expression of lncRNAs and their response to radiation and that lncRNAs may coordinate the tissue response to LDIR exposure via regulation of coding mRNAs.http://dx.doi.org/10.1155/2015/461038 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonathan Tang Yurong Huang David H. Nguyen Sylvain V. Costes Antoine M. Snijders Jian-Hua Mao |
spellingShingle |
Jonathan Tang Yurong Huang David H. Nguyen Sylvain V. Costes Antoine M. Snijders Jian-Hua Mao Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation International Journal of Genomics |
author_facet |
Jonathan Tang Yurong Huang David H. Nguyen Sylvain V. Costes Antoine M. Snijders Jian-Hua Mao |
author_sort |
Jonathan Tang |
title |
Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation |
title_short |
Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation |
title_full |
Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation |
title_fullStr |
Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation |
title_full_unstemmed |
Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation |
title_sort |
genetic background modulates lncrna-coordinated tissue response to low dose ionizing radiation |
publisher |
Hindawi Limited |
series |
International Journal of Genomics |
issn |
2314-436X 2314-4378 |
publishDate |
2015-01-01 |
description |
Long noncoding RNAs (lncRNAs) are emerging as key regulators of diverse cell functions and processes. However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. Here we used microarray profiling to determine lncRNA and mRNA expression in mammary glands of BALB/c and SPRET/EiJ mice after low-dose ionizing radiation (LDIR) exposure. We found that unirradiated mammary tissues of these strains differed significantly in baseline expressions of 290 lncRNAs. LDIR exposure (10 cGy) induced a significant change in the expression of many lncRNAs. The vast majority of lncRNAs identified to be differentially expressed after LDIR in either BALB/c or SPRET/EiJ had a significantly correlated expression pattern with at least one LDIR responsive mRNA. Functional analysis revealed that the response to LDIR in BALB/c mice is highly dynamic with enrichment for genes involved in tissue injury, inflammatory responses, and mammary gland development at 2, 4, and 8 weeks after LDIR, respectively. Our study demonstrates that genetic background strongly influences the expression of lncRNAs and their response to radiation and that lncRNAs may coordinate the tissue response to LDIR exposure via regulation of coding mRNAs. |
url |
http://dx.doi.org/10.1155/2015/461038 |
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