Patulin-Induced Suicidal Erythrocyte Death
Background: Patulin, the most common mycotoxin in apples and apple-derived products, triggers apoptosis and has thus been considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and...
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Cell Physiol Biochem Press GmbH & Co KG
2013-07-01
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doaj-c7e513a2a67341a790644de5619f1d6d2020-11-24T21:44:31ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-07-0132229129910.1159/000354437354437Patulin-Induced Suicidal Erythrocyte DeathAdrian LupescuKashif JilaniMohanad ZbidahFlorian LangBackground: Patulin, the most common mycotoxin in apples and apple-derived products, triggers apoptosis and has thus been considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by cell membrane scrambling leading to phosphatidylserine-exposure at the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored, whether exposure of human erythrocytes to patulin is followed by eryptosis. Methods: Forward scatter was measured to estimate cell volume, annexin V binding to detect phosphatidylserine-exposure, hemoglobin release to quantify hemolysis, and Fluo3-fuorescence to determine [Ca2+]i. Results: A 48 h exposure to patulin significantly increased [Ca2+]I (5 µM), significantly decreased forward scatter (5 µM) and significantly increased annexin-V-binding (2.5 µM). Patulin (10 µM) induced annexin-V-binding was virtually abrogated by removal of extracellular Ca2+. Conclusion: Patulin stimulates Ca2+ entry into erythrocytes, an effect triggering suicidal erythrocyte death or eryptosis.http://www.karger.com/Article/FullText/354437PhosphatidylserinePatulinCalciumCell volumeEryptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adrian Lupescu Kashif Jilani Mohanad Zbidah Florian Lang |
spellingShingle |
Adrian Lupescu Kashif Jilani Mohanad Zbidah Florian Lang Patulin-Induced Suicidal Erythrocyte Death Cellular Physiology and Biochemistry Phosphatidylserine Patulin Calcium Cell volume Eryptosis |
author_facet |
Adrian Lupescu Kashif Jilani Mohanad Zbidah Florian Lang |
author_sort |
Adrian Lupescu |
title |
Patulin-Induced Suicidal Erythrocyte Death |
title_short |
Patulin-Induced Suicidal Erythrocyte Death |
title_full |
Patulin-Induced Suicidal Erythrocyte Death |
title_fullStr |
Patulin-Induced Suicidal Erythrocyte Death |
title_full_unstemmed |
Patulin-Induced Suicidal Erythrocyte Death |
title_sort |
patulin-induced suicidal erythrocyte death |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2013-07-01 |
description |
Background: Patulin, the most common mycotoxin in apples and apple-derived products, triggers apoptosis and has thus been considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by cell membrane scrambling leading to phosphatidylserine-exposure at the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored, whether exposure of human erythrocytes to patulin is followed by eryptosis. Methods: Forward scatter was measured to estimate cell volume, annexin V binding to detect phosphatidylserine-exposure, hemoglobin release to quantify hemolysis, and Fluo3-fuorescence to determine [Ca2+]i. Results: A 48 h exposure to patulin significantly increased [Ca2+]I (5 µM), significantly decreased forward scatter (5 µM) and significantly increased annexin-V-binding (2.5 µM). Patulin (10 µM) induced annexin-V-binding was virtually abrogated by removal of extracellular Ca2+. Conclusion: Patulin stimulates Ca2+ entry into erythrocytes, an effect triggering suicidal erythrocyte death or eryptosis. |
topic |
Phosphatidylserine Patulin Calcium Cell volume Eryptosis |
url |
http://www.karger.com/Article/FullText/354437 |
work_keys_str_mv |
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