An “overlooked” habitat for thermophilic bacteria: the phyllosphere
Thermophilic microbes are present everywhere around us and their only known natural biotope is far away and most usually associated with geothermal energy. To answer this paradox, we explore the hypothesis that the phyllosphere (surface of leaves), due to its exposition to the sun, could well be a t...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Pensoft Publishers
2020-03-01
|
Series: | BioDiscovery |
Subjects: | |
Online Access: | https://biodiscovery.pensoft.net/article/47033/download/pdf/ |
id |
doaj-c7faf9d4ec32487e9d68ec7b2bac2030 |
---|---|
record_format |
Article |
spelling |
doaj-c7faf9d4ec32487e9d68ec7b2bac20302020-11-25T03:31:06ZengPensoft PublishersBioDiscovery2050-29662020-03-012311410.3897/biodiscovery.23.e4703347033An “overlooked” habitat for thermophilic bacteria: the phyllosphereJean Jacques Godon0Amandine Galès1Eric Latrille2Pornpimol Ouichanpagdee3Jean-Philippe Seyer4INRAE, Univ Montpellier, LBE, 102 avenue des EtangsINRAE, Univ Montpellier, LBEINRAE, Univ Montpellier, LBEDivision of Biotechnology, Faculty of Agro-Industry, Chiang Mai UniversityINRAE, Univ Montpellier, LBEThermophilic microbes are present everywhere around us and their only known natural biotope is far away and most usually associated with geothermal energy. To answer this paradox, we explore the hypothesis that the phyllosphere (surface of leaves), due to its exposition to the sun, could well be a thermophilic habitat for microbes and thus a source of thermophilic microbes growing around 50°C – 60°C. To support this hypothesis, we reviewed the heat sources on earth and associated microbial habitats, as well as the difficult identification of thermophilic microbes. We further present an experiment to show the presence and activity of thermophilic bacteria in the phyllosphere. Leaves were collected from eleven tree species from five locations on three continents belonging to three different biomes. On fresh leaves, 16S rDNA sequencing reveals the presence of 0.2 to 7% of clearly identified thermophilic bacteria. Moreover, after incubation at 55°C under aerobic and anaerobic conditions, 16S rDNA sequencing reveals the presence of 4 to 99% of clearly identified thermophilic bacteria. The accumulation of observations provides coherence to our hypothesis and allows the emergence of a new vision of leaves as a thermophilic biotope. We then propose a life cycle of microbes belonging to the thermophilic biotope associated with leaf surfaces.https://biodiscovery.pensoft.net/article/47033/download/pdf/phyllospherethermophilebiotopeairbornbacte |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jean Jacques Godon Amandine Galès Eric Latrille Pornpimol Ouichanpagdee Jean-Philippe Seyer |
spellingShingle |
Jean Jacques Godon Amandine Galès Eric Latrille Pornpimol Ouichanpagdee Jean-Philippe Seyer An “overlooked” habitat for thermophilic bacteria: the phyllosphere BioDiscovery phyllosphere thermophile biotope airborn bacte |
author_facet |
Jean Jacques Godon Amandine Galès Eric Latrille Pornpimol Ouichanpagdee Jean-Philippe Seyer |
author_sort |
Jean Jacques Godon |
title |
An “overlooked” habitat for thermophilic bacteria: the phyllosphere |
title_short |
An “overlooked” habitat for thermophilic bacteria: the phyllosphere |
title_full |
An “overlooked” habitat for thermophilic bacteria: the phyllosphere |
title_fullStr |
An “overlooked” habitat for thermophilic bacteria: the phyllosphere |
title_full_unstemmed |
An “overlooked” habitat for thermophilic bacteria: the phyllosphere |
title_sort |
“overlooked” habitat for thermophilic bacteria: the phyllosphere |
publisher |
Pensoft Publishers |
series |
BioDiscovery |
issn |
2050-2966 |
publishDate |
2020-03-01 |
description |
Thermophilic microbes are present everywhere around us and their only known natural biotope is far away and most usually associated with geothermal energy. To answer this paradox, we explore the hypothesis that the phyllosphere (surface of leaves), due to its exposition to the sun, could well be a thermophilic habitat for microbes and thus a source of thermophilic microbes growing around 50°C – 60°C. To support this hypothesis, we reviewed the heat sources on earth and associated microbial habitats, as well as the difficult identification of thermophilic microbes. We further present an experiment to show the presence and activity of thermophilic bacteria in the phyllosphere. Leaves were collected from eleven tree species from five locations on three continents belonging to three different biomes. On fresh leaves, 16S rDNA sequencing reveals the presence of 0.2 to 7% of clearly identified thermophilic bacteria. Moreover, after incubation at 55°C under aerobic and anaerobic conditions, 16S rDNA sequencing reveals the presence of 4 to 99% of clearly identified thermophilic bacteria. The accumulation of observations provides coherence to our hypothesis and allows the emergence of a new vision of leaves as a thermophilic biotope. We then propose a life cycle of microbes belonging to the thermophilic biotope associated with leaf surfaces. |
topic |
phyllosphere thermophile biotope airborn bacte |
url |
https://biodiscovery.pensoft.net/article/47033/download/pdf/ |
work_keys_str_mv |
AT jeanjacquesgodon anoverlookedhabitatforthermophilicbacteriathephyllosphere AT amandinegales anoverlookedhabitatforthermophilicbacteriathephyllosphere AT ericlatrille anoverlookedhabitatforthermophilicbacteriathephyllosphere AT pornpimolouichanpagdee anoverlookedhabitatforthermophilicbacteriathephyllosphere AT jeanphilippeseyer anoverlookedhabitatforthermophilicbacteriathephyllosphere AT jeanjacquesgodon overlookedhabitatforthermophilicbacteriathephyllosphere AT amandinegales overlookedhabitatforthermophilicbacteriathephyllosphere AT ericlatrille overlookedhabitatforthermophilicbacteriathephyllosphere AT pornpimolouichanpagdee overlookedhabitatforthermophilicbacteriathephyllosphere AT jeanphilippeseyer overlookedhabitatforthermophilicbacteriathephyllosphere |
_version_ |
1724573642637967360 |