Chemical compositions, A-glucosidase and A-amylase inhibitory activities of crude polysaccharides from the endodermis of shaddock (Citrus maxima)

• Main Author: Liu Guocong
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2012-01-01
• Series: Archives of Biological Sciences
• Subjects: Endodermis of shaddock; crude polysaccharides; chemical compositions; α-glucosidase and α-amylase inhibitory activities
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641201071L.pdf

The chemical composition of shaddock mainly includes polyphenols, proteins and polysaccharides. However, polysaccharides from shaddock materials have received much less consideration than polyphenols (Fellers et al., 1990). Herein the chemical compositions, α-glucosidase and α-amylase inhibitory activities of crude polysaccharides from the endodermis of shaddock were investigated. The exopolysaccharides (EPS) exhibited a broad and intense peak at 3300-3400 cm-1 that characterized the absorption of the hydroxyl group, and one weak C-H band at around 2941.3 cm-1 in the IR spectrum. The content of neutral sugars in EPS was determined as 37.16%. The content of acidic sugar in EPS was determined as 33.71%. EPS exhibited the highest content of neutral sugar. The content of proteins in EPS was 5.75%. The content of polyphenols was 6.52%. The EPS mainly consisted of four types of polysaccharides with molecular weights of 110 kD, 68 kD, 31 kD and 12 kD. The crude EPS showed significantly higher inhibitory effects on α-glucosidase and α-amylase (inhibition to 74.12% and 86.59%, respectively).