In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube
Prolonged use of implanted medical devices has been linked with device-associated infections. Since enteral tube feeding is increasing worldwide, the present study described the morphology of Candida albicans biofilms on the surface of silicone nasogastric tube (NGT) using fluorescence microscopy....
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Journal of Pure and Applied Microbiology
2019-12-01
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Series: | Journal of Pure and Applied Microbiology |
Subjects: | |
Online Access: | https://microbiologyjournal.org/in-vitro-resistance-pattern-of-selected-antifungal-azoles-against-candida-albicans-biofilms-on-silicone-nasogastric-tube/ |
Summary: | Prolonged use of implanted medical devices has been linked with device-associated infections. Since
enteral tube feeding is increasing worldwide, the present study described the morphology of Candida
albicans biofilms on the surface of silicone nasogastric tube (NGT) using fluorescence microscopy. With
the emergence of multidrug-resistant fungal infections, the present study assessed the susceptibility
profile of the biofilms to antifungal azoles namely fluconazole, miconazole, voriconazole, and
posaconazole using the standard disc diffusion method. Microscopic studies of C. albicans biofilms
revealed a complex heterogeneous structure with yeast cells and hyphal elements entrenched within a
polysaccharide matrix. Planktonic C. albicans cells remained susceptible with posaconazole, fluconazole,
miconazole, and voriconazole. However, the fungal biofilms exhibited resistance with miconazole. There
was a significant reduction in the zone of inhibition on the 24-h, 48-h, and 72-h biofilm formation with
posaconazole, voriconazole, and fluconazole, respectively. Kinetic investigation on C. albicans biofilm
with posaconazole revealed a zero-order kinetic profile (R2
=0.9774) whereas with voriconazole exhibited
a first-order kinetic profile (R2
=0.9974). These findings can possibly provide information regarding the
resistance of fungal biofilms with antifungal azoles. Demonstration of common biofilm features will
extend the findings of this study beyond fungi to polymicrobial infections, as new information and
insights will influence several disciplines ranging from environmental microbiology to pharmaceutical
drug design intended for biofilm-associated infections. |
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ISSN: | 0973-7510 2581-690X |