In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube
Prolonged use of implanted medical devices has been linked with device-associated infections. Since enteral tube feeding is increasing worldwide, the present study described the morphology of Candida albicans biofilms on the surface of silicone nasogastric tube (NGT) using fluorescence microscopy....
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doaj-c8274bdcb1b94a4cb0a7422ba31a44562021-10-02T17:24:11ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-12-011341881189110.22207/JPAM.13.4.01In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric TubeDerick Erl P. Sumalapao0Pauline Bridgette D. Salazar1Frederico Martin D. Alegre2Nelson R. Villarante3Nina G. Gloriani41 Department of Epidemiology and Biostatistics, College of Public Health, University of the Philippines Manila, Manila, Philippines. 2 Biology Department, College of Science, De La Salle University, Manila, Philippines. 2 Biology Department, College of Science, De La Salle University, Manila, Philippines. 3 College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines. 2 Biology Department, College of Science, De La Salle University, Manila, Philippines. 3 College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines. 4 Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Philippines.5 Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila, Philippines.Prolonged use of implanted medical devices has been linked with device-associated infections. Since enteral tube feeding is increasing worldwide, the present study described the morphology of Candida albicans biofilms on the surface of silicone nasogastric tube (NGT) using fluorescence microscopy. With the emergence of multidrug-resistant fungal infections, the present study assessed the susceptibility profile of the biofilms to antifungal azoles namely fluconazole, miconazole, voriconazole, and posaconazole using the standard disc diffusion method. Microscopic studies of C. albicans biofilms revealed a complex heterogeneous structure with yeast cells and hyphal elements entrenched within a polysaccharide matrix. Planktonic C. albicans cells remained susceptible with posaconazole, fluconazole, miconazole, and voriconazole. However, the fungal biofilms exhibited resistance with miconazole. There was a significant reduction in the zone of inhibition on the 24-h, 48-h, and 72-h biofilm formation with posaconazole, voriconazole, and fluconazole, respectively. Kinetic investigation on C. albicans biofilm with posaconazole revealed a zero-order kinetic profile (R2 =0.9774) whereas with voriconazole exhibited a first-order kinetic profile (R2 =0.9974). These findings can possibly provide information regarding the resistance of fungal biofilms with antifungal azoles. Demonstration of common biofilm features will extend the findings of this study beyond fungi to polymicrobial infections, as new information and insights will influence several disciplines ranging from environmental microbiology to pharmaceutical drug design intended for biofilm-associated infections.https://microbiologyjournal.org/in-vitro-resistance-pattern-of-selected-antifungal-azoles-against-candida-albicans-biofilms-on-silicone-nasogastric-tube/posaconazolefluconazolemiconazolevoriconazolefeeding tubekinetic equations |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Derick Erl P. Sumalapao Pauline Bridgette D. Salazar Frederico Martin D. Alegre Nelson R. Villarante Nina G. Gloriani |
spellingShingle |
Derick Erl P. Sumalapao Pauline Bridgette D. Salazar Frederico Martin D. Alegre Nelson R. Villarante Nina G. Gloriani In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube Journal of Pure and Applied Microbiology posaconazole fluconazole miconazole voriconazole feeding tube kinetic equations |
author_facet |
Derick Erl P. Sumalapao Pauline Bridgette D. Salazar Frederico Martin D. Alegre Nelson R. Villarante Nina G. Gloriani |
author_sort |
Derick Erl P. Sumalapao |
title |
In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube |
title_short |
In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube |
title_full |
In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube |
title_fullStr |
In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube |
title_full_unstemmed |
In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube |
title_sort |
in vitro resistance pattern of selected antifungal azoles against candida albicans biofilms on silicone nasogastric tube |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2019-12-01 |
description |
Prolonged use of implanted medical devices has been linked with device-associated infections. Since
enteral tube feeding is increasing worldwide, the present study described the morphology of Candida
albicans biofilms on the surface of silicone nasogastric tube (NGT) using fluorescence microscopy. With
the emergence of multidrug-resistant fungal infections, the present study assessed the susceptibility
profile of the biofilms to antifungal azoles namely fluconazole, miconazole, voriconazole, and
posaconazole using the standard disc diffusion method. Microscopic studies of C. albicans biofilms
revealed a complex heterogeneous structure with yeast cells and hyphal elements entrenched within a
polysaccharide matrix. Planktonic C. albicans cells remained susceptible with posaconazole, fluconazole,
miconazole, and voriconazole. However, the fungal biofilms exhibited resistance with miconazole. There
was a significant reduction in the zone of inhibition on the 24-h, 48-h, and 72-h biofilm formation with
posaconazole, voriconazole, and fluconazole, respectively. Kinetic investigation on C. albicans biofilm
with posaconazole revealed a zero-order kinetic profile (R2
=0.9774) whereas with voriconazole exhibited
a first-order kinetic profile (R2
=0.9974). These findings can possibly provide information regarding the
resistance of fungal biofilms with antifungal azoles. Demonstration of common biofilm features will
extend the findings of this study beyond fungi to polymicrobial infections, as new information and
insights will influence several disciplines ranging from environmental microbiology to pharmaceutical
drug design intended for biofilm-associated infections. |
topic |
posaconazole fluconazole miconazole voriconazole feeding tube kinetic equations |
url |
https://microbiologyjournal.org/in-vitro-resistance-pattern-of-selected-antifungal-azoles-against-candida-albicans-biofilms-on-silicone-nasogastric-tube/ |
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