Microbiota of Cow’s Milk with Udder Pathologies

• Main Authors: Mariya V. Gryaznova, Mikhail Y. Syromyatnikov, Yulia D. Dvoretskaya, Sergey A. Solodskikh, Nikolay T. Klimov, Vitaliy I. Mikhalev, Vitaliy I. Zimnikov, Evgeniy V. Mikhaylov, Vasily N. Popov
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Microorganisms
• Subjects: microbiome; udder pathologies; sequencing; mastitis; 16S rRNA
• Online Access: https://www.mdpi.com/2076-2607/9/9/1974

Mastitis is the most common disease for cattle, causing great economic losses for the global dairy industry. Recent studies indicate the multi-agent and microbiome diversity of this disease. To understand the nature of mastitis and investigate the role of the microbiome in the development of pathologies in the udder of bovines, we performed NGS sequencing of the 16S rRNA gene of cow’s milk with pathologies of the udder. The obtained data show a significant increase in the <i>Cutibacterium</i>, <i>Blautia</i>, <i>Clostridium sensu stricto 2</i>, <i>Staphylococcus</i>, <i>Streptococcus</i> and <i>Microbacterium</i> genera for groups of cows with udder pathologies. Increasing relative abundance of the <i>Staphylococcus</i> and <i>Streptococcus</i> genera was associated with subclinical mastitis. Our data show that a relative increase in abundance of the <i>Staphylococcus</i> and <i>Microbacterium</i> genera may be an early sign of infection. We have shown, for the first time, an increase in the <i>Colidextribacter</i>, <i>Paeniclostridium</i> and <i>Turicibacter</i> genera in groups of cows with mastitis. These results expand our understanding of the role of the microbiome in the development of bovine mastitis.