Microbiota of Cow’s Milk with Udder Pathologies
Mastitis is the most common disease for cattle, causing great economic losses for the global dairy industry. Recent studies indicate the multi-agent and microbiome diversity of this disease. To understand the nature of mastitis and investigate the role of the microbiome in the development of patholo...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-09-01
|
Series: | Microorganisms |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2607/9/9/1974 |
id |
doaj-c83bd140951b47fa931afe0a58f290d5 |
---|---|
record_format |
Article |
spelling |
doaj-c83bd140951b47fa931afe0a58f290d52021-09-26T00:44:23ZengMDPI AGMicroorganisms2076-26072021-09-0191974197410.3390/microorganisms9091974Microbiota of Cow’s Milk with Udder PathologiesMariya V. Gryaznova0Mikhail Y. Syromyatnikov1Yulia D. Dvoretskaya2Sergey A. Solodskikh3Nikolay T. Klimov4Vitaliy I. Mikhalev5Vitaliy I. Zimnikov6Evgeniy V. Mikhaylov7Vasily N. Popov8Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaFSBSI All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, 394061 Voronezh, RussiaFSBSI All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, 394061 Voronezh, RussiaFSBSI All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, 394061 Voronezh, RussiaFSBSI All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, 394061 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaMastitis is the most common disease for cattle, causing great economic losses for the global dairy industry. Recent studies indicate the multi-agent and microbiome diversity of this disease. To understand the nature of mastitis and investigate the role of the microbiome in the development of pathologies in the udder of bovines, we performed NGS sequencing of the 16S rRNA gene of cow’s milk with pathologies of the udder. The obtained data show a significant increase in the <i>Cutibacterium</i>, <i>Blautia</i>, <i>Clostridium sensu stricto 2</i>, <i>Staphylococcus</i>, <i>Streptococcus</i> and <i>Microbacterium</i> genera for groups of cows with udder pathologies. Increasing relative abundance of the <i>Staphylococcus</i> and <i>Streptococcus</i> genera was associated with subclinical mastitis. Our data show that a relative increase in abundance of the <i>Staphylococcus</i> and <i>Microbacterium</i> genera may be an early sign of infection. We have shown, for the first time, an increase in the <i>Colidextribacter</i>, <i>Paeniclostridium</i> and <i>Turicibacter</i> genera in groups of cows with mastitis. These results expand our understanding of the role of the microbiome in the development of bovine mastitis.https://www.mdpi.com/2076-2607/9/9/1974microbiomeudder pathologiessequencingmastitis16S rRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariya V. Gryaznova Mikhail Y. Syromyatnikov Yulia D. Dvoretskaya Sergey A. Solodskikh Nikolay T. Klimov Vitaliy I. Mikhalev Vitaliy I. Zimnikov Evgeniy V. Mikhaylov Vasily N. Popov |
spellingShingle |
Mariya V. Gryaznova Mikhail Y. Syromyatnikov Yulia D. Dvoretskaya Sergey A. Solodskikh Nikolay T. Klimov Vitaliy I. Mikhalev Vitaliy I. Zimnikov Evgeniy V. Mikhaylov Vasily N. Popov Microbiota of Cow’s Milk with Udder Pathologies Microorganisms microbiome udder pathologies sequencing mastitis 16S rRNA |
author_facet |
Mariya V. Gryaznova Mikhail Y. Syromyatnikov Yulia D. Dvoretskaya Sergey A. Solodskikh Nikolay T. Klimov Vitaliy I. Mikhalev Vitaliy I. Zimnikov Evgeniy V. Mikhaylov Vasily N. Popov |
author_sort |
Mariya V. Gryaznova |
title |
Microbiota of Cow’s Milk with Udder Pathologies |
title_short |
Microbiota of Cow’s Milk with Udder Pathologies |
title_full |
Microbiota of Cow’s Milk with Udder Pathologies |
title_fullStr |
Microbiota of Cow’s Milk with Udder Pathologies |
title_full_unstemmed |
Microbiota of Cow’s Milk with Udder Pathologies |
title_sort |
microbiota of cow’s milk with udder pathologies |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-09-01 |
description |
Mastitis is the most common disease for cattle, causing great economic losses for the global dairy industry. Recent studies indicate the multi-agent and microbiome diversity of this disease. To understand the nature of mastitis and investigate the role of the microbiome in the development of pathologies in the udder of bovines, we performed NGS sequencing of the 16S rRNA gene of cow’s milk with pathologies of the udder. The obtained data show a significant increase in the <i>Cutibacterium</i>, <i>Blautia</i>, <i>Clostridium sensu stricto 2</i>, <i>Staphylococcus</i>, <i>Streptococcus</i> and <i>Microbacterium</i> genera for groups of cows with udder pathologies. Increasing relative abundance of the <i>Staphylococcus</i> and <i>Streptococcus</i> genera was associated with subclinical mastitis. Our data show that a relative increase in abundance of the <i>Staphylococcus</i> and <i>Microbacterium</i> genera may be an early sign of infection. We have shown, for the first time, an increase in the <i>Colidextribacter</i>, <i>Paeniclostridium</i> and <i>Turicibacter</i> genera in groups of cows with mastitis. These results expand our understanding of the role of the microbiome in the development of bovine mastitis. |
topic |
microbiome udder pathologies sequencing mastitis 16S rRNA |
url |
https://www.mdpi.com/2076-2607/9/9/1974 |
work_keys_str_mv |
AT mariyavgryaznova microbiotaofcowsmilkwithudderpathologies AT mikhailysyromyatnikov microbiotaofcowsmilkwithudderpathologies AT yuliaddvoretskaya microbiotaofcowsmilkwithudderpathologies AT sergeyasolodskikh microbiotaofcowsmilkwithudderpathologies AT nikolaytklimov microbiotaofcowsmilkwithudderpathologies AT vitaliyimikhalev microbiotaofcowsmilkwithudderpathologies AT vitaliyizimnikov microbiotaofcowsmilkwithudderpathologies AT evgeniyvmikhaylov microbiotaofcowsmilkwithudderpathologies AT vasilynpopov microbiotaofcowsmilkwithudderpathologies |
_version_ |
1716869962957586432 |