Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells
The anti-melanogenic ingredient from aqueous extracts of soy milk fermented by Lactobacillus plantarum TWK10 (TWK10) was isolated and identified as uracil. In addition, the mechanism by which uracil inhibits melanogenesis in α-melanocyte stimulating hormone (α-MSH)-stimulated B16 melanoma cells was...
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doaj-c878219a257b4ee78002deba64848fea2021-04-29T04:44:37ZengElsevierJournal of Functional Foods1756-46462015-08-0117314327Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cellsChia-Jen Chang0Ru-Yu Dai1Yann-Lii Leu2Tsung-Yu Tsai3Department of Food Science, Fu Jen Catholic University, Taipei, TaiwanDepartment of Food Science, Fu Jen Catholic University, Taipei, TaiwanGraduate Institute of Natural Products, Chang Gung University, Taoyuan, TaiwanDepartment of Food Science, Fu Jen Catholic University, Taipei, Taiwan; Corresponding author. No. 510, Zhongzheng Rd., Xinzhuang Dist., 24205, New Taipei City, Taiwan. Tel.: +886 2 2905 2539; fax: +886 2 2905 2540.The anti-melanogenic ingredient from aqueous extracts of soy milk fermented by Lactobacillus plantarum TWK10 (TWK10) was isolated and identified as uracil. In addition, the mechanism by which uracil inhibits melanogenesis in α-melanocyte stimulating hormone (α-MSH)-stimulated B16 melanoma cells was investigated. Concentrations of uracil less than 100 µg/mL had no cytotoxic effect on α-MSH-stimulated B16 melanoma cells, nor any effect on their cell cycle distribution, but did reduce melanin content in the cells. The mechanisms by which uracil inhibited melanin production included down-regulating the transcription of the gene encoding melanocortin 1 receptor, decreasing the phosphorylation of cyclic adenosine monophosphate response element-binding protein, and repressing the expression of microphthalmia-associated transcription factor (MITF). In addition, a uracil-mediated suppression of phosphorylation of p38 mitogen-activated protein kinase, leading to concomitant decrease in MITF levels, was observed. The reduction in MITF levels leads to decreases in the expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2, resulting in lower melanin production in α-MSH-stimulated B16 melanoma cells. These results indicate that uracil, derived from aqueous extracts of TWK10-fermented soy milk, is a substance that is safe to use on B16 melanoma cells and has anti-melanogenic effects. It may be used to develop functional foods and cosmetics, and to increase the range of applications of traditional agricultural products.http://www.sciencedirect.com/science/article/pii/S1756464615002601CREBLactobacillus plantarum TWK10MITFSoy milkUracil |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chia-Jen Chang Ru-Yu Dai Yann-Lii Leu Tsung-Yu Tsai |
spellingShingle |
Chia-Jen Chang Ru-Yu Dai Yann-Lii Leu Tsung-Yu Tsai Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells Journal of Functional Foods CREB Lactobacillus plantarum TWK10 MITF Soy milk Uracil |
author_facet |
Chia-Jen Chang Ru-Yu Dai Yann-Lii Leu Tsung-Yu Tsai |
author_sort |
Chia-Jen Chang |
title |
Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells |
title_short |
Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells |
title_full |
Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells |
title_fullStr |
Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells |
title_full_unstemmed |
Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells |
title_sort |
effects of the melanogenic inhibitor, uracil, derived from lactobacillus plantarum twk10-fermented soy milk on anti-melanogenesis in b16f0 mouse melanoma cells |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2015-08-01 |
description |
The anti-melanogenic ingredient from aqueous extracts of soy milk fermented by Lactobacillus plantarum TWK10 (TWK10) was isolated and identified as uracil. In addition, the mechanism by which uracil inhibits melanogenesis in α-melanocyte stimulating hormone (α-MSH)-stimulated B16 melanoma cells was investigated. Concentrations of uracil less than 100 µg/mL had no cytotoxic effect on α-MSH-stimulated B16 melanoma cells, nor any effect on their cell cycle distribution, but did reduce melanin content in the cells. The mechanisms by which uracil inhibited melanin production included down-regulating the transcription of the gene encoding melanocortin 1 receptor, decreasing the phosphorylation of cyclic adenosine monophosphate response element-binding protein, and repressing the expression of microphthalmia-associated transcription factor (MITF). In addition, a uracil-mediated suppression of phosphorylation of p38 mitogen-activated protein kinase, leading to concomitant decrease in MITF levels, was observed. The reduction in MITF levels leads to decreases in the expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2, resulting in lower melanin production in α-MSH-stimulated B16 melanoma cells. These results indicate that uracil, derived from aqueous extracts of TWK10-fermented soy milk, is a substance that is safe to use on B16 melanoma cells and has anti-melanogenic effects. It may be used to develop functional foods and cosmetics, and to increase the range of applications of traditional agricultural products. |
topic |
CREB Lactobacillus plantarum TWK10 MITF Soy milk Uracil |
url |
http://www.sciencedirect.com/science/article/pii/S1756464615002601 |
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