Modified ultrafast Papanicolaou staining technique: A comparative study
Introduction: Ultrafast Papanicolaou stain (UFP) was introduced as a hybrid of Romanowsky and Papanicolaou (PAP) stain. It enhances the quality and reduces the time. In the present study, a modified staining technique was adapted where Gill's Hematoxylin was replaced by Harris Hematoxylin. Aim...
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doaj-c8ba28bf2c294e32b7b2b2ff1dbbdd6b2020-11-24T23:33:42ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712017-01-0134314915310.4103/JOC.JOC_23_16Modified ultrafast Papanicolaou staining technique: A comparative studyMoni ThakurVenkateswara Rao GuttikondaIntroduction: Ultrafast Papanicolaou stain (UFP) was introduced as a hybrid of Romanowsky and Papanicolaou (PAP) stain. It enhances the quality and reduces the time. In the present study, a modified staining technique was adapted where Gill's Hematoxylin was replaced by Harris Hematoxylin. Aims: The aim of the study was to assess the use of the modified ultrafast Papanicolaou (MUFP) stain for fine needle aspiration cytology (FNAC) of head and neck swellings in comparison with the routine PAP stain, hematoxylin and eosin (H and E), and Giemsa. Materials and Methods: Forty FNACs of head and neck swellings were collected. FNAC procedure was performed by standard method; two smears were fixed in 95% propanol and stained with PAP and H and E. Two smears were air dried, 1 was stained with Giemsa, and 1 was rehydrated with normal saline, fixed in alcoholic formalin, and stained with MUFP. Four parameters were considered and scored background, cell morphology, nuclear staining, and overall staining pattern). Results: The quality of MUFP smears were better when compared to routine PAP, H and E, and Giemsa, and was statistically significant by Wilcoxon matched pair test. Conclusions: MUFP stain in comparison to routine PAP, H and E, and Giemsa provides an excellent and suitable alterative in cytological staining for the study of various organs.http://www.jcytol.org/article.asp?issn=0970-9371;year=2017;volume=34;issue=3;spage=149;epage=153;aulast=ThakurFine needle aspiration cytologygiemsahematoxylin and eosinmodified ultrafast papanicolaou stainpapanicolaou stain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Moni Thakur Venkateswara Rao Guttikonda |
spellingShingle |
Moni Thakur Venkateswara Rao Guttikonda Modified ultrafast Papanicolaou staining technique: A comparative study Journal of Cytology Fine needle aspiration cytology giemsa hematoxylin and eosin modified ultrafast papanicolaou stain papanicolaou stain |
author_facet |
Moni Thakur Venkateswara Rao Guttikonda |
author_sort |
Moni Thakur |
title |
Modified ultrafast Papanicolaou staining technique: A comparative study |
title_short |
Modified ultrafast Papanicolaou staining technique: A comparative study |
title_full |
Modified ultrafast Papanicolaou staining technique: A comparative study |
title_fullStr |
Modified ultrafast Papanicolaou staining technique: A comparative study |
title_full_unstemmed |
Modified ultrafast Papanicolaou staining technique: A comparative study |
title_sort |
modified ultrafast papanicolaou staining technique: a comparative study |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Cytology |
issn |
0970-9371 |
publishDate |
2017-01-01 |
description |
Introduction: Ultrafast Papanicolaou stain (UFP) was introduced as a hybrid of Romanowsky and Papanicolaou (PAP) stain. It enhances the quality and reduces the time. In the present study, a modified staining technique was adapted where Gill's Hematoxylin was replaced by Harris Hematoxylin.
Aims: The aim of the study was to assess the use of the modified ultrafast Papanicolaou (MUFP) stain for fine needle aspiration cytology (FNAC) of head and neck swellings in comparison with the routine PAP stain, hematoxylin and eosin (H and E), and Giemsa.
Materials and Methods: Forty FNACs of head and neck swellings were collected. FNAC procedure was performed by standard method; two smears were fixed in 95% propanol and stained with PAP and H and E. Two smears were air dried, 1 was stained with Giemsa, and 1 was rehydrated with normal saline, fixed in alcoholic formalin, and stained with MUFP. Four parameters were considered and scored background, cell morphology, nuclear staining, and overall staining pattern).
Results: The quality of MUFP smears were better when compared to routine PAP, H and E, and Giemsa, and was statistically significant by Wilcoxon matched pair test.
Conclusions: MUFP stain in comparison to routine PAP, H and E, and Giemsa provides an excellent and suitable alterative in cytological staining for the study of various organs. |
topic |
Fine needle aspiration cytology giemsa hematoxylin and eosin modified ultrafast papanicolaou stain papanicolaou stain |
url |
http://www.jcytol.org/article.asp?issn=0970-9371;year=2017;volume=34;issue=3;spage=149;epage=153;aulast=Thakur |
work_keys_str_mv |
AT monithakur modifiedultrafastpapanicolaoustainingtechniqueacomparativestudy AT venkateswararaoguttikonda modifiedultrafastpapanicolaoustainingtechniqueacomparativestudy |
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1725531047040385024 |