Cyclin K as a Direct Transcriptional Target of the p53 Tumor Suppressor

Cyclin K as a Direct Transcriptional Target of the p53 Tumor Suppressor

Cyclin K, a newly recognized member of the “transcription” cyclin family, may play a dual role by regulating CDK and transcription. Using cDNA microarray technology, we found that cyclin K mRNA was dramatically increased in U373MG, a glioblastoma cell line deficient in wild-type p53, in the presenc...

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Bibliographic Details
Main Authors: Toshiki Mori, Yoshio Anazawa, Kuniko Matsui, Seisuke Fukuda, Yusuke Nakamura, Hirofumi Arakawa
Format: Article
Language:English
Published: Elsevier 2002-01-01
Series:Neoplasia: An International Journal for Oncology Research
Subjects:
cyclin K
cell cycle
p53
p53 target gene
cDNA microarray
Online Access:http://www.sciencedirect.com/science/article/pii/S1476558602800215
Description
Summary:Cyclin K, a newly recognized member of the “transcription” cyclin family, may play a dual role by regulating CDK and transcription. Using cDNA microarray technology, we found that cyclin K mRNA was dramatically increased in U373MG, a glioblastoma cell line deficient in wild-type p53, in the presence of exogenous p53. An electrophoretic mobility-shift assay showed that a potential p53-binding site (p53BS) in intron 1 of the cyclin K gene could indeed bind to p53 protein. Moreover, a heterologous reporter assay revealed that the p53BS possessed p53-dependent transcriptional activity. Colony-formation assays indicated that overexpression of cyclin K suppressed growth of T98G, U373MG and SW480 cells. The results suggested that cyclin K may play a role in regulating the cell cycle or apoptosis after being targeted for transcription by p53.
ISSN:1476-5586
1522-8002

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