Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

Summary: Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for...

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Main Authors: Zhaoyuan Liu, Yaqi Gu, Amanda Shin, Shuangyan Zhang, Florent Ginhoux
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300162
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spelling doaj-c8cb64c0950a47c39067c24e4b3d48072020-11-25T04:07:13ZengElsevierSTAR Protocols2666-16672020-06-0111100029Analysis of Myeloid Cells in Mouse Tissues with Flow CytometryZhaoyuan Liu0Yaqi Gu1Amanda Shin2Shuangyan Zhang3Florent Ginhoux4Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Corresponding authorShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 138648, Singapore; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore, Singapore; Corresponding authorSummary: Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166720300162
collection DOAJ
language English
format Article
sources DOAJ
author Zhaoyuan Liu
Yaqi Gu
Amanda Shin
Shuangyan Zhang
Florent Ginhoux
spellingShingle Zhaoyuan Liu
Yaqi Gu
Amanda Shin
Shuangyan Zhang
Florent Ginhoux
Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
STAR Protocols
author_facet Zhaoyuan Liu
Yaqi Gu
Amanda Shin
Shuangyan Zhang
Florent Ginhoux
author_sort Zhaoyuan Liu
title Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_short Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_full Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_fullStr Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_full_unstemmed Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_sort analysis of myeloid cells in mouse tissues with flow cytometry
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-06-01
description Summary: Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).
url http://www.sciencedirect.com/science/article/pii/S2666166720300162
work_keys_str_mv AT zhaoyuanliu analysisofmyeloidcellsinmousetissueswithflowcytometry
AT yaqigu analysisofmyeloidcellsinmousetissueswithflowcytometry
AT amandashin analysisofmyeloidcellsinmousetissueswithflowcytometry
AT shuangyanzhang analysisofmyeloidcellsinmousetissueswithflowcytometry
AT florentginhoux analysisofmyeloidcellsinmousetissueswithflowcytometry
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