Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran
Background: The erythrocyte binding antigen 175 kDa (EBA-175) gene is located on chromosome 7. It encodes protein that binds to specific receptor glycophorin A on the erythrocyte surface during invasion. It has a dimorphic nature (FCR3 and CAMP). This study was designed to determine the distributi...
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Tehran University of Medical Sciences
2009-06-01
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doaj-c8dc1b5f84034a429cc7758236d052562021-04-02T13:05:07ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2009-06-0142Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of IranA Heidari0H Keshavarz1S Dittrich2T Jelinek3 Background: The erythrocyte binding antigen 175 kDa (EBA-175) gene is located on chromosome 7. It encodes protein that binds to specific receptor glycophorin A on the erythrocyte surface during invasion. It has a dimorphic nature (FCR3 and CAMP). This study was designed to determine the distribution of EBA-175 alleles of Plasmodium falciparum in the southeast of Iran Methods: We used the nested PCR method with specific primers, which improves the two fragments of the EBA-175 gene. Sixty eight microscopically positive blood samples were collected from the infected falciparum malaria subjects in the southeast of Iran. Results: In this study which marks the first one in Iran, CAMP strains (714 bp) and FCR-3 strains (795 bp) were found in 14 (37.8%) and 23 (62.2%) in the originally Iranian subjects and in 10 (32.3%) and 19 (61.3%) Pakistani infected migrants respectively. Two migrant cases (6.4%) had mix CAMP/FCR-3 infection. Conclusion: The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in Iran. There was no significant correlation between one of the EBA-175 alleles and the subject group in the mentioned region. This distributional pattern should be considered in designing to control P. falciparum malaria in the region. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/93MalariaPlasmodium falciparumErythrocyte binding antigen-175Iran |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A Heidari H Keshavarz S Dittrich T Jelinek |
spellingShingle |
A Heidari H Keshavarz S Dittrich T Jelinek Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran Iranian Journal of Parasitology Malaria Plasmodium falciparum Erythrocyte binding antigen-175 Iran |
author_facet |
A Heidari H Keshavarz S Dittrich T Jelinek |
author_sort |
A Heidari |
title |
Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran |
title_short |
Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran |
title_full |
Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran |
title_fullStr |
Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran |
title_full_unstemmed |
Allelic Dimorphism of the Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) Gene in the South-east of Iran |
title_sort |
allelic dimorphism of the plasmodium falciparum erythrocyte binding antigen-175 (eba-175) gene in the south-east of iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2009-06-01 |
description |
Background: The erythrocyte binding antigen 175 kDa (EBA-175) gene is located on chromosome 7. It encodes protein that binds to specific receptor glycophorin A on the erythrocyte surface during invasion. It has a dimorphic nature (FCR3 and CAMP). This study was designed to determine the distribution of EBA-175 alleles of Plasmodium falciparum in the southeast of Iran
Methods: We used the nested PCR method with specific primers, which improves the two fragments of the EBA-175 gene. Sixty eight microscopically positive blood samples were collected from the infected falciparum malaria subjects in the southeast of Iran.
Results: In this study which marks the first one in Iran, CAMP strains (714 bp) and FCR-3 strains (795 bp) were found in 14 (37.8%) and 23 (62.2%) in the originally Iranian subjects and in 10 (32.3%) and 19 (61.3%) Pakistani infected migrants respectively. Two migrant cases (6.4%) had mix CAMP/FCR-3 infection.
Conclusion: The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in Iran. There was no significant correlation between one of the EBA-175 alleles and the subject group in the mentioned region. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.
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topic |
Malaria Plasmodium falciparum Erythrocyte binding antigen-175 Iran |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/93 |
work_keys_str_mv |
AT aheidari allelicdimorphismoftheplasmodiumfalciparumerythrocytebindingantigen175eba175geneinthesoutheastofiran AT hkeshavarz allelicdimorphismoftheplasmodiumfalciparumerythrocytebindingantigen175eba175geneinthesoutheastofiran AT sdittrich allelicdimorphismoftheplasmodiumfalciparumerythrocytebindingantigen175eba175geneinthesoutheastofiran AT tjelinek allelicdimorphismoftheplasmodiumfalciparumerythrocytebindingantigen175eba175geneinthesoutheastofiran |
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