Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells
Anaplastic large cell lymphoma (ALCL) and classical Hodgkin lymphoma (cHL) are lymphomas that contain CD30-expressing tumor cells and have numerous pathological similarities. Whereas ALCL is usually diagnosed at an advanced stage, cHL more frequently presents with localized disease. The aim of the p...
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doaj-c8e67e775209432ab22e35f518f314322020-11-25T02:27:40ZengMDPI AGCancers2072-66942019-10-011110148410.3390/cancers11101484cancers11101484Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma CellsOlga Goncharova0Nadine Flinner1Julia Bein2Claudia Döring3Emmanuel Donnadieu4Sandy Rikirsch5Marco Herling6Ralf Küppers7Martin-Leo Hansmann8Sylvia Hartmann9Dr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyInstitute of Informatics/Frankfurt Institute for Advanced Studies, Goethe University, 60438 Frankfurt am Main, GermanyDr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyDr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyInserm, U1016, Institut Cochin, CNRS, UMR8104 and Université Paris Descartes, F-75014 Paris, FranceDr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyThe Laboratory of Lymphocyte Signaling and Oncoproteome, Department I of Internal Medicine, Center for Integrated Oncology (CIO) Aachen-Bonn-Cologne-Duesseldorf, CECAD and CMMC, University of Cologne, 50937 Cologne, GermanyInstitute of Cell Biology (Cancer Research), University of Duisburg-Essen, 45122 Essen, GermanyDr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyDr. Senckenberg Institute of Pathology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyAnaplastic large cell lymphoma (ALCL) and classical Hodgkin lymphoma (cHL) are lymphomas that contain CD30-expressing tumor cells and have numerous pathological similarities. Whereas ALCL is usually diagnosed at an advanced stage, cHL more frequently presents with localized disease. The aim of the present study was to elucidate the mechanisms underlying the different clinical presentation of ALCL and cHL. Chemokine and chemokine receptor expression were similar in primary ALCL and cHL cases apart from the known overexpression of the chemokines CCL17 and CCL22 in the Hodgkin and Reed-Sternberg (HRS) cells of cHL. Consistent with the overexpression of these chemokines, primary cHL cases encountered a significantly denser T cell microenvironment than ALCL. Additionally to differences in the interaction with their microenvironment, cHL cell lines presented a lower and less efficient intrinsic cell motility than ALCL cell lines, as assessed by time-lapse microscopy in a collagen gel and transwell migration assays. We thus propose that the combination of impaired basal cell motility and differences in the interaction with the microenvironment hamper the dissemination of HRS cells in cHL when compared with the tumor cells of ALCL.https://www.mdpi.com/2072-6694/11/10/1484anaplastic large cell lymphomadisseminationrosetting t cellsclassical hodgkin lymphomachemokine receptorscell motilitysegmentationimage analysisgene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olga Goncharova Nadine Flinner Julia Bein Claudia Döring Emmanuel Donnadieu Sandy Rikirsch Marco Herling Ralf Küppers Martin-Leo Hansmann Sylvia Hartmann |
spellingShingle |
Olga Goncharova Nadine Flinner Julia Bein Claudia Döring Emmanuel Donnadieu Sandy Rikirsch Marco Herling Ralf Küppers Martin-Leo Hansmann Sylvia Hartmann Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells Cancers anaplastic large cell lymphoma dissemination rosetting t cells classical hodgkin lymphoma chemokine receptors cell motility segmentation image analysis gene expression |
author_facet |
Olga Goncharova Nadine Flinner Julia Bein Claudia Döring Emmanuel Donnadieu Sandy Rikirsch Marco Herling Ralf Küppers Martin-Leo Hansmann Sylvia Hartmann |
author_sort |
Olga Goncharova |
title |
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells |
title_short |
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells |
title_full |
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells |
title_fullStr |
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells |
title_full_unstemmed |
Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells |
title_sort |
migration properties distinguish tumor cells of classical hodgkin lymphoma from anaplastic large cell lymphoma cells |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2019-10-01 |
description |
Anaplastic large cell lymphoma (ALCL) and classical Hodgkin lymphoma (cHL) are lymphomas that contain CD30-expressing tumor cells and have numerous pathological similarities. Whereas ALCL is usually diagnosed at an advanced stage, cHL more frequently presents with localized disease. The aim of the present study was to elucidate the mechanisms underlying the different clinical presentation of ALCL and cHL. Chemokine and chemokine receptor expression were similar in primary ALCL and cHL cases apart from the known overexpression of the chemokines CCL17 and CCL22 in the Hodgkin and Reed-Sternberg (HRS) cells of cHL. Consistent with the overexpression of these chemokines, primary cHL cases encountered a significantly denser T cell microenvironment than ALCL. Additionally to differences in the interaction with their microenvironment, cHL cell lines presented a lower and less efficient intrinsic cell motility than ALCL cell lines, as assessed by time-lapse microscopy in a collagen gel and transwell migration assays. We thus propose that the combination of impaired basal cell motility and differences in the interaction with the microenvironment hamper the dissemination of HRS cells in cHL when compared with the tumor cells of ALCL. |
topic |
anaplastic large cell lymphoma dissemination rosetting t cells classical hodgkin lymphoma chemokine receptors cell motility segmentation image analysis gene expression |
url |
https://www.mdpi.com/2072-6694/11/10/1484 |
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