LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome
Abstract Background TMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. Its role in other ovary diseases is unknown. This study explored its role in polycystic ovary syndrome (PCOS). Methods Follicular fluid was extracted from both PCOS patients and controls. The levels of TMPO-...
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doaj-c8e9def8cee740428bc790f87cd8dc892021-08-01T11:35:07ZengBMCJournal of Ovarian Research1757-22152021-07-011411610.1186/s13048-021-00848-3LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndromeFang Hou0Jie Li1Jie Peng2Zhenghua Teng3Jun Feng4Weiwei Xia5Department of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalDepartment of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalDepartment of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalDepartment of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalDepartment of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalDepartment of Obstetrics and Gynecology, Suzhou Wuzhong People’s HospitalAbstract Background TMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. Its role in other ovary diseases is unknown. This study explored its role in polycystic ovary syndrome (PCOS). Methods Follicular fluid was extracted from both PCOS patients and controls. The levels of TMPO-AS1 and mature and premature miR-335-5p were analyzed by RT-qPCR. The role of TMPO-AS1 in regulating miR-355-5p maturation in granulosa-like tumor (KGN) cells was analyzed by overexpression experiments. The interaction between TMPO-AS1 and premature miR-335-5p was analyzed by RNA pull-down assay. The subcellular location of TMPO-AS1 in KGN cells was analyzed by nuclear fractionation assay. The role of TMPO-AS1 and miR-335-5p in KGN cell proliferation was analyzed by BrdU assay. Results TMPO-AS1 was increased in PCOS, while mature miR-355-5p was decreased in PCOS. TMPO-AS1 overexpression decreased mature miR-355-5p level but increased premature miR-355-5p. TMPO-AS1 was localized in both nucleus and cytoplasm. TMPO-AS1 directly interacted with premature miR-355-5p in KGN cells. TMPO-AS1 increased KGN cell proliferation while miR-355-5p decreased cell proliferation. The co-transfection assay showed that TMPO-AS1 reduced the suppressive effects of miR-355-5p on cell proliferation. Conclusions TMPO-AS1 might suppress miR-335-5p maturation to participate in PCOS.https://doi.org/10.1186/s13048-021-00848-3Polycystic ovary syndromeTMPO-AS1miR-335-5pMaturation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Hou Jie Li Jie Peng Zhenghua Teng Jun Feng Weiwei Xia |
spellingShingle |
Fang Hou Jie Li Jie Peng Zhenghua Teng Jun Feng Weiwei Xia LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome Journal of Ovarian Research Polycystic ovary syndrome TMPO-AS1 miR-335-5p Maturation |
author_facet |
Fang Hou Jie Li Jie Peng Zhenghua Teng Jun Feng Weiwei Xia |
author_sort |
Fang Hou |
title |
LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome |
title_short |
LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome |
title_full |
LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome |
title_fullStr |
LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome |
title_full_unstemmed |
LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome |
title_sort |
lncrna tmpo-as1 suppresses the maturation of mir-335-5p to participate in polycystic ovary syndrome |
publisher |
BMC |
series |
Journal of Ovarian Research |
issn |
1757-2215 |
publishDate |
2021-07-01 |
description |
Abstract Background TMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. Its role in other ovary diseases is unknown. This study explored its role in polycystic ovary syndrome (PCOS). Methods Follicular fluid was extracted from both PCOS patients and controls. The levels of TMPO-AS1 and mature and premature miR-335-5p were analyzed by RT-qPCR. The role of TMPO-AS1 in regulating miR-355-5p maturation in granulosa-like tumor (KGN) cells was analyzed by overexpression experiments. The interaction between TMPO-AS1 and premature miR-335-5p was analyzed by RNA pull-down assay. The subcellular location of TMPO-AS1 in KGN cells was analyzed by nuclear fractionation assay. The role of TMPO-AS1 and miR-335-5p in KGN cell proliferation was analyzed by BrdU assay. Results TMPO-AS1 was increased in PCOS, while mature miR-355-5p was decreased in PCOS. TMPO-AS1 overexpression decreased mature miR-355-5p level but increased premature miR-355-5p. TMPO-AS1 was localized in both nucleus and cytoplasm. TMPO-AS1 directly interacted with premature miR-355-5p in KGN cells. TMPO-AS1 increased KGN cell proliferation while miR-355-5p decreased cell proliferation. The co-transfection assay showed that TMPO-AS1 reduced the suppressive effects of miR-355-5p on cell proliferation. Conclusions TMPO-AS1 might suppress miR-335-5p maturation to participate in PCOS. |
topic |
Polycystic ovary syndrome TMPO-AS1 miR-335-5p Maturation |
url |
https://doi.org/10.1186/s13048-021-00848-3 |
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