Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A

Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A

A new assay for 3-hydroxy-3-methylglutaryl CoA reductase (mevalonate: NADP oxidoreductase [acylating CoA], EC 1.1.1.34) is based upon the measurement of released coenzyme A (SH) during the reduction of 3-hydroxy-3-methylglutaryl CoA to mevalonate. Coenzyme A was measured in the presence of dithiothr...

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Bibliographic Details
Main Authors:	Frank H. Hulcher, Wayne H. Oleson
Format:	Article
Language:	English
Published:	Elsevier 1973-11-01
Series:	Journal of Lipid Research
Subjects:	pigeon liver yeast microsomes 3-hydroxy-3-methylglutaryl CoA deacylase sulfhydryl group arsenite-dithiol complex enzyme kinetics
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520368437

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