Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy
Objectives: CD4+Foxp3+ regulatory T-cells (Treg) are known to accumulate under certain pathological conditions. This study was conducted to evaluate the characteristics of and dynamic changes in Treg cells in chronic hepatitis C (CHC) patients during antiviral therapy. Methods: One hundred and forty...
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doaj-c90f4d2e12ca4474be76d96584f1e52f2020-11-24T23:50:17ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112016-04-0145C51210.1016/j.ijid.2016.02.006Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapyZhiqin Li0Yu Ping1Zujiang Yu2Meng Wang3Dongli Yue4Zhen Zhang5Jianbin Li6Bin Zhang7Xuezhong Shi8Yi Zhang9Department of Infectious Disease, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaDepartment of Infectious Disease, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaHenan Red Cross Blood Center, Zhengzhou, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaDepartment of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou 450000, Henan, ChinaBiotherapy Center, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou 450052, Henan, ChinaObjectives: CD4+Foxp3+ regulatory T-cells (Treg) are known to accumulate under certain pathological conditions. This study was conducted to evaluate the characteristics of and dynamic changes in Treg cells in chronic hepatitis C (CHC) patients during antiviral therapy. Methods: One hundred and forty-five subjects were enrolled in this study, including 105 CHC patients and 40 healthy donors. The phenotypes and functions of Treg cells were analyzed by flow cytometry. Results: A significant elevation in Treg cells was observed in the peripheral blood of CHC patients compared with healthy donors. Interestingly, compared with non-suppressive Treg (non-Treg) and resting Treg (rTreg) cells, activated Treg (aTreg) cells expressed higher levels of ectonucleotidase, CD39, and CD73. After treatment with interferon alpha (IFN-α) and ribavirin (RBV) in vitro, the frequencies of total Treg cells and aTreg cells in peripheral blood mononuclear cells (PBMC), as well as the levels of transforming growth factor beta (TGF-β) secreted by aTreg and non-Treg cells, were significantly decreased. Importantly, it was found that levels of aTreg cells in patients with a sustained virological response (SVR) were lower than in relapsed patients, suggesting that a high frequency of aTreg cells might be associated with a poor clinical outcome in HCV infection. Conclusion: These results demonstrate a decreasing trend in aTreg cells, which express higher levels of CD39, CD73, and TGF-β, in SVR patients during antiviral therapy.http://www.sciencedirect.com/science/article/pii/S1201971216000266Treg cellsHCV antiviral therapyIFN-αRibavirin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiqin Li Yu Ping Zujiang Yu Meng Wang Dongli Yue Zhen Zhang Jianbin Li Bin Zhang Xuezhong Shi Yi Zhang |
spellingShingle |
Zhiqin Li Yu Ping Zujiang Yu Meng Wang Dongli Yue Zhen Zhang Jianbin Li Bin Zhang Xuezhong Shi Yi Zhang Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy International Journal of Infectious Diseases Treg cells HCV antiviral therapy IFN-α Ribavirin |
author_facet |
Zhiqin Li Yu Ping Zujiang Yu Meng Wang Dongli Yue Zhen Zhang Jianbin Li Bin Zhang Xuezhong Shi Yi Zhang |
author_sort |
Zhiqin Li |
title |
Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy |
title_short |
Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy |
title_full |
Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy |
title_fullStr |
Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy |
title_full_unstemmed |
Dynamic changes in CD45RA−Foxp3high regulatory T-cells in chronic hepatitis C patients during antiviral therapy |
title_sort |
dynamic changes in cd45ra−foxp3high regulatory t-cells in chronic hepatitis c patients during antiviral therapy |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 1878-3511 |
publishDate |
2016-04-01 |
description |
Objectives: CD4+Foxp3+ regulatory T-cells (Treg) are known to accumulate under certain pathological conditions. This study was conducted to evaluate the characteristics of and dynamic changes in Treg cells in chronic hepatitis C (CHC) patients during antiviral therapy.
Methods: One hundred and forty-five subjects were enrolled in this study, including 105 CHC patients and 40 healthy donors. The phenotypes and functions of Treg cells were analyzed by flow cytometry.
Results: A significant elevation in Treg cells was observed in the peripheral blood of CHC patients compared with healthy donors. Interestingly, compared with non-suppressive Treg (non-Treg) and resting Treg (rTreg) cells, activated Treg (aTreg) cells expressed higher levels of ectonucleotidase, CD39, and CD73. After treatment with interferon alpha (IFN-α) and ribavirin (RBV) in vitro, the frequencies of total Treg cells and aTreg cells in peripheral blood mononuclear cells (PBMC), as well as the levels of transforming growth factor beta (TGF-β) secreted by aTreg and non-Treg cells, were significantly decreased. Importantly, it was found that levels of aTreg cells in patients with a sustained virological response (SVR) were lower than in relapsed patients, suggesting that a high frequency of aTreg cells might be associated with a poor clinical outcome in HCV infection.
Conclusion: These results demonstrate a decreasing trend in aTreg cells, which express higher levels of CD39, CD73, and TGF-β, in SVR patients during antiviral therapy. |
topic |
Treg cells HCV antiviral therapy IFN-α Ribavirin |
url |
http://www.sciencedirect.com/science/article/pii/S1201971216000266 |
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