A novel source of hospital microorganisms in healthcare settings
Background. Most healthcare-associated infections (HAI) develop due to a colonization of patients and healthcare workers by hospital strains of pathogens. The aim to study was to assess whether the dust within the health facilities can harbor microorganisms acting as a reservoir of HAIs.Materials an...
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Central Research Institute for Epidemiology
2021-07-01
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doaj-c91a442694a04c02822ecafa60c747062021-07-28T13:07:54ZrusCentral Research Institute for Epidemiology Журнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132021-07-0198326627510.36233/0372-9311-120736A novel source of hospital microorganisms in healthcare settingsE. A. Chezganova0O. S. Efimova1V. M. Sakharova2A. R. Efimova3S. A. Sozinov4Z. R. Ismagilov5E. B. Brusina6Kemerovo State Medical UniversityInstitute of Coal Chemistry and Material Science of the Siberian Branch of the Russian Academy of SciencesResearch Institute for Complex Issues of Cardiovascular DiseasesKemerovo Regional Center for Hygiene and EpidemiologyInstitute of Coal Chemistry and Material Science of the Siberian Branch of the Russian Academy of SciencesInstitute of Coal Chemistry and Material Science of the Siberian Branch of the Russian Academy of SciencesKemerovo State Medical UniversityBackground. Most healthcare-associated infections (HAI) develop due to a colonization of patients and healthcare workers by hospital strains of pathogens. The aim to study was to assess whether the dust within the health facilities can harbor microorganisms acting as a reservoir of HAIs.Materials and methods. Dust samples collected in the air ducts and ventilation grilles of health facilities underwent a detailed physicochemical analysis by means of scanning electron microscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, and high-temperature catalytic oxidation. Bacterial and viral diversity was investigated using an automated biochemical analyzer and polymerase chain reaction, respectively. Investigation of the microenvironment included detection of biofilms using a catalase indicator and quantification of viable microorganisms per 1 m3 air.Results. Dust from the hospital ventilation grilles and air ducts was contaminated with microorganisms in 71.13% of cases. Strikingly, multidrug-resistant and biofilm-forming strains have been found in 69.4% and 48.0% of samples, respectively. The total viable count before and after opening doors and windows was 276 and 462 colony-forming units/m3 respectively (p = 0.046). Biodiversity was represented by 21 genera of microorganisms which were consistently detected upon 6 months of follow-up. All samples contained a nanosized particulate matter. Chemical elements comprising dust were carbon (16.26–50.69%), oxygen (20.02–37.50%), nitrogen (1.59–25.03%), hydrogen (2.03–6.67%), sulfur (0.15–2.38%), calcium (0.19–7.49%), silicon (0.21–4.64%), chlorine (0.05–2.83%), sodium (0.07–1.86%), aluminum (0.36–1.78%), iron (0.08–1.61%), magnesium (0.11–1.40%), potassium (0.04–0.85%), and phosphorus (0.04–0.81%).Discussion. A wide range of multidrug-resistant strains of bacteria, detected in a hospital particulate matter with a diverse chemical composition, indicates the persistence of HAI-causing pathogens in the hospital environment.Conclusion. Dust from the ventilation grilles and adjacent air ducts should be considered as an additional reservoir of multidrug-resistant strains of bacteria in the healthcare settings.https://microbiol.elpub.ru/jour/article/view/1039reservoirmicroorganismsparticulate matterhealthcare-associated infections |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
E. A. Chezganova O. S. Efimova V. M. Sakharova A. R. Efimova S. A. Sozinov Z. R. Ismagilov E. B. Brusina |
spellingShingle |
E. A. Chezganova O. S. Efimova V. M. Sakharova A. R. Efimova S. A. Sozinov Z. R. Ismagilov E. B. Brusina A novel source of hospital microorganisms in healthcare settings Журнал микробиологии, эпидемиологии и иммунобиологии reservoir microorganisms particulate matter healthcare-associated infections |
author_facet |
E. A. Chezganova O. S. Efimova V. M. Sakharova A. R. Efimova S. A. Sozinov Z. R. Ismagilov E. B. Brusina |
author_sort |
E. A. Chezganova |
title |
A novel source of hospital microorganisms in healthcare settings |
title_short |
A novel source of hospital microorganisms in healthcare settings |
title_full |
A novel source of hospital microorganisms in healthcare settings |
title_fullStr |
A novel source of hospital microorganisms in healthcare settings |
title_full_unstemmed |
A novel source of hospital microorganisms in healthcare settings |
title_sort |
novel source of hospital microorganisms in healthcare settings |
publisher |
Central Research Institute for Epidemiology |
series |
Журнал микробиологии, эпидемиологии и иммунобиологии |
issn |
0372-9311 2686-7613 |
publishDate |
2021-07-01 |
description |
Background. Most healthcare-associated infections (HAI) develop due to a colonization of patients and healthcare workers by hospital strains of pathogens. The aim to study was to assess whether the dust within the health facilities can harbor microorganisms acting as a reservoir of HAIs.Materials and methods. Dust samples collected in the air ducts and ventilation grilles of health facilities underwent a detailed physicochemical analysis by means of scanning electron microscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, and high-temperature catalytic oxidation. Bacterial and viral diversity was investigated using an automated biochemical analyzer and polymerase chain reaction, respectively. Investigation of the microenvironment included detection of biofilms using a catalase indicator and quantification of viable microorganisms per 1 m3 air.Results. Dust from the hospital ventilation grilles and air ducts was contaminated with microorganisms in 71.13% of cases. Strikingly, multidrug-resistant and biofilm-forming strains have been found in 69.4% and 48.0% of samples, respectively. The total viable count before and after opening doors and windows was 276 and 462 colony-forming units/m3 respectively (p = 0.046). Biodiversity was represented by 21 genera of microorganisms which were consistently detected upon 6 months of follow-up. All samples contained a nanosized particulate matter. Chemical elements comprising dust were carbon (16.26–50.69%), oxygen (20.02–37.50%), nitrogen (1.59–25.03%), hydrogen (2.03–6.67%), sulfur (0.15–2.38%), calcium (0.19–7.49%), silicon (0.21–4.64%), chlorine (0.05–2.83%), sodium (0.07–1.86%), aluminum (0.36–1.78%), iron (0.08–1.61%), magnesium (0.11–1.40%), potassium (0.04–0.85%), and phosphorus (0.04–0.81%).Discussion. A wide range of multidrug-resistant strains of bacteria, detected in a hospital particulate matter with a diverse chemical composition, indicates the persistence of HAI-causing pathogens in the hospital environment.Conclusion. Dust from the ventilation grilles and adjacent air ducts should be considered as an additional reservoir of multidrug-resistant strains of bacteria in the healthcare settings. |
topic |
reservoir microorganisms particulate matter healthcare-associated infections |
url |
https://microbiol.elpub.ru/jour/article/view/1039 |
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