In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita
Abstract Background Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2019-12-01
|
Series: | Bulletin of the National Research Centre |
Subjects: | |
Online Access: | https://doi.org/10.1186/s42269-019-0200-0 |
id |
doaj-c932954267b54d51ae9ab79348b3e9c3 |
---|---|
record_format |
Article |
spelling |
doaj-c932954267b54d51ae9ab79348b3e9c32020-12-06T12:03:28ZengSpringerOpenBulletin of the National Research Centre2522-83072019-12-014311710.1186/s42269-019-0200-0In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognitaGaziea Mohamed Soliman0Hoda Hussein Ameen1Shadia Mohamed Abdel-Aziz2Ghada M. El-Sayed3Agricultural and Biological Division, Department of Plant Pathology, National Research CentreAgricultural and Biological Division, Department of Plant Pathology, National Research CentreGenetic Engineering and Biotechnology Division, Microbial Genetic Department, National Research CentreGenetic Engineering and Biotechnology Division, Microbial Genetic Department, National Research CentreAbstract Background Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their nematicidal activity against Meloidogyne incognita J2 mortality and egg hatching in in vitro test. Results Lytic Rhizobacteria were isolated from soil samples adhering to tomato plant roots from different localities at Giza governorate, Egypt. Six bacterial isolates, exhibited high efficacy against root-knot nematodes, were identified based on the analysis of the 16S rRNA gene sequence as Pseudomonas aeruginosa, Paenibacillus polymyxa, Lysinibacillus sphaericus, Bacillus cereus, Bacillus subtilis, and Achromobacter xylosoxidans. These strains showed high production of chitinase, chitosanase, and protease using colloidal chitin and soluble chitosan as carbon sources. Two strains, Paenibacillus polymyxa and Bacillus subtilis, produced the highest levels of chitinase in the media. Except for Pseudomonas aeruginosa, all the bacterial strains produced high levels of chitosanase. Conclusion The results revealed that high amounts of protease were, however, secreted by Pseudomonas aeruginosa as compared to the other strains. In in vitro tests, all the bacterial culture filtrates potentially displayed nematicidal effect in M. incognita egg hatching and an obvious increase in J2 mortality as compared to control. Paenibacillus polymyxa caused 100% juvenile mortality followed by Bacillus subtilis 97.25%, Bacillus cereus 94%, Achromobacter xylosoxidans 93%, Lysinibacillus sphaericus 92%, and Pseudomonas aeruginosa 84.29% after 48 h of exposure, as compared to control.https://doi.org/10.1186/s42269-019-0200-0Rhizobacteria16S rRNALytic enzymesBiocontrolMeloidogyne incognita |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaziea Mohamed Soliman Hoda Hussein Ameen Shadia Mohamed Abdel-Aziz Ghada M. El-Sayed |
spellingShingle |
Gaziea Mohamed Soliman Hoda Hussein Ameen Shadia Mohamed Abdel-Aziz Ghada M. El-Sayed In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita Bulletin of the National Research Centre Rhizobacteria 16S rRNA Lytic enzymes Biocontrol Meloidogyne incognita |
author_facet |
Gaziea Mohamed Soliman Hoda Hussein Ameen Shadia Mohamed Abdel-Aziz Ghada M. El-Sayed |
author_sort |
Gaziea Mohamed Soliman |
title |
In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita |
title_short |
In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita |
title_full |
In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita |
title_fullStr |
In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita |
title_full_unstemmed |
In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita |
title_sort |
in vitro evaluation of some isolated bacteria against the plant parasite nematode meloidogyne incognita |
publisher |
SpringerOpen |
series |
Bulletin of the National Research Centre |
issn |
2522-8307 |
publishDate |
2019-12-01 |
description |
Abstract Background Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their nematicidal activity against Meloidogyne incognita J2 mortality and egg hatching in in vitro test. Results Lytic Rhizobacteria were isolated from soil samples adhering to tomato plant roots from different localities at Giza governorate, Egypt. Six bacterial isolates, exhibited high efficacy against root-knot nematodes, were identified based on the analysis of the 16S rRNA gene sequence as Pseudomonas aeruginosa, Paenibacillus polymyxa, Lysinibacillus sphaericus, Bacillus cereus, Bacillus subtilis, and Achromobacter xylosoxidans. These strains showed high production of chitinase, chitosanase, and protease using colloidal chitin and soluble chitosan as carbon sources. Two strains, Paenibacillus polymyxa and Bacillus subtilis, produced the highest levels of chitinase in the media. Except for Pseudomonas aeruginosa, all the bacterial strains produced high levels of chitosanase. Conclusion The results revealed that high amounts of protease were, however, secreted by Pseudomonas aeruginosa as compared to the other strains. In in vitro tests, all the bacterial culture filtrates potentially displayed nematicidal effect in M. incognita egg hatching and an obvious increase in J2 mortality as compared to control. Paenibacillus polymyxa caused 100% juvenile mortality followed by Bacillus subtilis 97.25%, Bacillus cereus 94%, Achromobacter xylosoxidans 93%, Lysinibacillus sphaericus 92%, and Pseudomonas aeruginosa 84.29% after 48 h of exposure, as compared to control. |
topic |
Rhizobacteria 16S rRNA Lytic enzymes Biocontrol Meloidogyne incognita |
url |
https://doi.org/10.1186/s42269-019-0200-0 |
work_keys_str_mv |
AT gazieamohamedsoliman invitroevaluationofsomeisolatedbacteriaagainsttheplantparasitenematodemeloidogyneincognita AT hodahusseinameen invitroevaluationofsomeisolatedbacteriaagainsttheplantparasitenematodemeloidogyneincognita AT shadiamohamedabdelaziz invitroevaluationofsomeisolatedbacteriaagainsttheplantparasitenematodemeloidogyneincognita AT ghadamelsayed invitroevaluationofsomeisolatedbacteriaagainsttheplantparasitenematodemeloidogyneincognita |
_version_ |
1724399368827568128 |