Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model
Red blood cells (RBCs) have a well-defined lifespan, indicating a mechanism by which senescent cells of a certain age are removed from circulation. However, the specifics by which senescent cells are recognized and removed are poorly understood. There are multiple competing hypotheses for this proce...
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doaj-c942496390ec43aa84174d6c0658876c2020-11-25T00:11:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-10-01810.3389/fimmu.2017.01425295095Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine ModelKrystalyn E. Hudson0Karen de Wolski1Linda M. Kapp2Amanda L. Richards3Matthew J. Schniederjan4James C. Zimring5James C. Zimring6James C. Zimring7Bloodworks Northwest Research Institute, Seattle, WA, United StatesBloodworks Northwest Research Institute, Seattle, WA, United StatesBloodworks Northwest Research Institute, Seattle, WA, United StatesBloodworks Northwest Research Institute, Seattle, WA, United StatesDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, United StatesBloodworks Northwest Research Institute, Seattle, WA, United StatesDepartment of Laboratory Medicine, Division of Hematology, University of Washington, Seattle, WA, United StatesDepartment of Internal Medicine, Division of Hematology, University of Washington, Seattle, WA, United StatesRed blood cells (RBCs) have a well-defined lifespan, indicating a mechanism by which senescent cells of a certain age are removed from circulation. However, the specifics by which senescent cells are recognized and removed are poorly understood. There are multiple competing hypotheses for this process, perhaps the most commonly cited is that senescent RBCs expose neoantigens [or senescent antigen(s)] that are then recognized by naturally occurring antibodies, which opsonize the senescent cells and result in clearance from circulation. While there are a large volume of published data to indicate that older RBCs accumulate increased levels of antibody on their surface, to the best of our knowledge, the causal role of such antibodies in clearance has not been rigorously assessed. In the current report, we demonstrate that RBC lifespan and clearance patterns are not altered in mice deficient in antibodies, in C3 protein, or missing both. These data demonstrate that neither antibody nor C3 is required for clearance of senescent RBCs, and questions if they are even involved, in a murine model of RBC lifespan.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01425/fullred blood cell clearancecomplement C3red blood cell lifespanantibodiessenescent antigennaturally occurring antibodies |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Krystalyn E. Hudson Karen de Wolski Linda M. Kapp Amanda L. Richards Matthew J. Schniederjan James C. Zimring James C. Zimring James C. Zimring |
spellingShingle |
Krystalyn E. Hudson Karen de Wolski Linda M. Kapp Amanda L. Richards Matthew J. Schniederjan James C. Zimring James C. Zimring James C. Zimring Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model Frontiers in Immunology red blood cell clearance complement C3 red blood cell lifespan antibodies senescent antigen naturally occurring antibodies |
author_facet |
Krystalyn E. Hudson Karen de Wolski Linda M. Kapp Amanda L. Richards Matthew J. Schniederjan James C. Zimring James C. Zimring James C. Zimring |
author_sort |
Krystalyn E. Hudson |
title |
Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model |
title_short |
Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model |
title_full |
Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model |
title_fullStr |
Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model |
title_full_unstemmed |
Antibodies to Senescent Antigen and C3 Are Not Required for Normal Red Blood Cell Lifespan in a Murine Model |
title_sort |
antibodies to senescent antigen and c3 are not required for normal red blood cell lifespan in a murine model |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-10-01 |
description |
Red blood cells (RBCs) have a well-defined lifespan, indicating a mechanism by which senescent cells of a certain age are removed from circulation. However, the specifics by which senescent cells are recognized and removed are poorly understood. There are multiple competing hypotheses for this process, perhaps the most commonly cited is that senescent RBCs expose neoantigens [or senescent antigen(s)] that are then recognized by naturally occurring antibodies, which opsonize the senescent cells and result in clearance from circulation. While there are a large volume of published data to indicate that older RBCs accumulate increased levels of antibody on their surface, to the best of our knowledge, the causal role of such antibodies in clearance has not been rigorously assessed. In the current report, we demonstrate that RBC lifespan and clearance patterns are not altered in mice deficient in antibodies, in C3 protein, or missing both. These data demonstrate that neither antibody nor C3 is required for clearance of senescent RBCs, and questions if they are even involved, in a murine model of RBC lifespan. |
topic |
red blood cell clearance complement C3 red blood cell lifespan antibodies senescent antigen naturally occurring antibodies |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01425/full |
work_keys_str_mv |
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