Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test
As the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO<sub>2</sub> NPs, 25 and...
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doaj-c961eb36061a45119ceec72d65ae9e6e2020-11-24T23:56:42ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-02-0120488210.3390/ijms20040882ijms20040882Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity TestSandra Vranic0Yasuhito Shimada1Sahoko Ichihara2Masayuki Kimata3Wenting Wu4Toshio Tanaka5Sonja Boland6Lang Tran7Gaku Ichihara8Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanDepartment of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu 514-8572, JapanDepartment of Environmental and Preventive Medicine, Jichi Medical University School of Medicine, Shimotsuke 329-0498, JapanDepartment of Human Functional Genomics, Life Science Research Center, Mie University, Tsu 514-8572, JapanDepartment of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanDepartment of Systems Pharmacology, Mie University Graduate School of Medicine, Tsu 514-8572, JapanUnit of Functional and Adaptive Biology (BFA), Laboratory of Molecular and Cellular Responses to Xenobiotics, CNRS UMR 8251, Universite Paris Diderot, Sorbonne Paris Cite, 75013 Paris, FranceInstitute of Occupational Medicine, Research Avenue North, Riccarton, Edinburgh EH14 4AP, UKDepartment of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanAs the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO<sub>2</sub> NPs, 25 and 115 nm, with neutral surface charge or with different surface functionalization, rendering them positively or negatively charged, in order to predict the effect of NPs in humans. We performed a zebrafish embryo toxicity test (ZFET) by exposing the embryos to SiO<sub>2</sub> NPs starting from six hours post fertilization (hpf). Survival rate, hatching time, and gross morphological changes were assessed at 12, 24, 36, 48, 60, and 72 hpf. We evaluated the effect of NPs on angiogenesis by counting the number of sub-intestinal vessels between the second and seventh intersegmental vessels and gene expression analysis of vascular endothelial growth factor (VEGF) and VEGF receptors at 72 hpf. SiO<sub>2</sub> NPs did not show any adverse effects on survival rate, hatching time, gross morphology, or physiological angiogenesis. We found that SiO<sub>2</sub> NPs were trapped by the chorion up until to the hatching stage. After chemical removal of the chorion (dechorionation), positively surface-charged SiO<sub>2</sub> NPs (25 nm) significantly reduced the survival rate of the fish compared to the control group. These results indicate that zebrafish chorion acts as a physical barrier against SiO<sub>2</sub> NPs, and removing the chorions in ZFET might be necessary for evaluation of toxicity of NPs.https://www.mdpi.com/1422-0067/20/4/882silica nanoparticlessurface functionalizationzebrafishembryo acute toxicity testvascularizationbio-distributiondechorionation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandra Vranic Yasuhito Shimada Sahoko Ichihara Masayuki Kimata Wenting Wu Toshio Tanaka Sonja Boland Lang Tran Gaku Ichihara |
spellingShingle |
Sandra Vranic Yasuhito Shimada Sahoko Ichihara Masayuki Kimata Wenting Wu Toshio Tanaka Sonja Boland Lang Tran Gaku Ichihara Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test International Journal of Molecular Sciences silica nanoparticles surface functionalization zebrafish embryo acute toxicity test vascularization bio-distribution dechorionation |
author_facet |
Sandra Vranic Yasuhito Shimada Sahoko Ichihara Masayuki Kimata Wenting Wu Toshio Tanaka Sonja Boland Lang Tran Gaku Ichihara |
author_sort |
Sandra Vranic |
title |
Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test |
title_short |
Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test |
title_full |
Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test |
title_fullStr |
Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test |
title_full_unstemmed |
Toxicological Evaluation of SiO<sub>2</sub> Nanoparticles by Zebrafish Embryo Toxicity Test |
title_sort |
toxicological evaluation of sio<sub>2</sub> nanoparticles by zebrafish embryo toxicity test |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-02-01 |
description |
As the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO<sub>2</sub> NPs, 25 and 115 nm, with neutral surface charge or with different surface functionalization, rendering them positively or negatively charged, in order to predict the effect of NPs in humans. We performed a zebrafish embryo toxicity test (ZFET) by exposing the embryos to SiO<sub>2</sub> NPs starting from six hours post fertilization (hpf). Survival rate, hatching time, and gross morphological changes were assessed at 12, 24, 36, 48, 60, and 72 hpf. We evaluated the effect of NPs on angiogenesis by counting the number of sub-intestinal vessels between the second and seventh intersegmental vessels and gene expression analysis of vascular endothelial growth factor (VEGF) and VEGF receptors at 72 hpf. SiO<sub>2</sub> NPs did not show any adverse effects on survival rate, hatching time, gross morphology, or physiological angiogenesis. We found that SiO<sub>2</sub> NPs were trapped by the chorion up until to the hatching stage. After chemical removal of the chorion (dechorionation), positively surface-charged SiO<sub>2</sub> NPs (25 nm) significantly reduced the survival rate of the fish compared to the control group. These results indicate that zebrafish chorion acts as a physical barrier against SiO<sub>2</sub> NPs, and removing the chorions in ZFET might be necessary for evaluation of toxicity of NPs. |
topic |
silica nanoparticles surface functionalization zebrafish embryo acute toxicity test vascularization bio-distribution dechorionation |
url |
https://www.mdpi.com/1422-0067/20/4/882 |
work_keys_str_mv |
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