Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria
<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> &l...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-01-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Online Access: | http://www.ann-clinmicrob.com/content/9/1/1 |
| Summary: | <p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> <p>Methods</p> <p>Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.</p> <p>Results</p> <p>By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an <it>E. coli </it>host. Sequence analysis of the recombinant plasmid revealed <it>bla</it><sub>SHV-12 </sub>associated to the insertion sequence, IS<it>26</it>.</p> <p>Conclusion</p> <p>This is the first study demonstrated the occurrence of SHV-12 in Nigeria.</p> |
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| ISSN: | 1476-0711 |