Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria

• Main Authors: Budak Fatma, Kolayli Fetiye, Torol Sinem, Fashae Kayode, Kasap Murat, Vahaboglu Haluk
• Format: Article
• Language: English
• Published: BMC 2010-01-01
• Series: Annals of Clinical Microbiology and Antimicrobials
• Online Access: http://www.ann-clinmicrob.com/content/9/1/1

<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> <p>Methods</p> <p>Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.</p> <p>Results</p> <p>By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an <it>E. coli </it>host. Sequence analysis of the recombinant plasmid revealed <it>bla</it>_SHV-12associated to the insertion sequence, IS<it>26</it>.</p> <p>Conclusion</p> <p>This is the first study demonstrated the occurrence of SHV-12 in Nigeria.</p>