Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria
<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> &l...
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doaj-c976c9cb603f4f16a5a79467ade7cd092020-11-24T21:14:32ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112010-01-0191110.1186/1476-0711-9-1Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in NigeriaBudak FatmaKolayli FetiyeTorol SinemFashae KayodeKasap MuratVahaboglu Haluk<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> <p>Methods</p> <p>Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.</p> <p>Results</p> <p>By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an <it>E. coli </it>host. Sequence analysis of the recombinant plasmid revealed <it>bla</it><sub>SHV-12 </sub>associated to the insertion sequence, IS<it>26</it>.</p> <p>Conclusion</p> <p>This is the first study demonstrated the occurrence of SHV-12 in Nigeria.</p> http://www.ann-clinmicrob.com/content/9/1/1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Budak Fatma Kolayli Fetiye Torol Sinem Fashae Kayode Kasap Murat Vahaboglu Haluk |
spellingShingle |
Budak Fatma Kolayli Fetiye Torol Sinem Fashae Kayode Kasap Murat Vahaboglu Haluk Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria Annals of Clinical Microbiology and Antimicrobials |
author_facet |
Budak Fatma Kolayli Fetiye Torol Sinem Fashae Kayode Kasap Murat Vahaboglu Haluk |
author_sort |
Budak Fatma |
title |
Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria |
title_short |
Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria |
title_full |
Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria |
title_fullStr |
Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria |
title_full_unstemmed |
Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria |
title_sort |
characterization of esbl (shv-12) producing clinical isolate of <it>enterobacter aerogenes </it>from a tertiary care hospital in nigeria |
publisher |
BMC |
series |
Annals of Clinical Microbiology and Antimicrobials |
issn |
1476-0711 |
publishDate |
2010-01-01 |
description |
<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> <p>Methods</p> <p>Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.</p> <p>Results</p> <p>By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an <it>E. coli </it>host. Sequence analysis of the recombinant plasmid revealed <it>bla</it><sub>SHV-12 </sub>associated to the insertion sequence, IS<it>26</it>.</p> <p>Conclusion</p> <p>This is the first study demonstrated the occurrence of SHV-12 in Nigeria.</p> |
url |
http://www.ann-clinmicrob.com/content/9/1/1 |
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