Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor.
Small-diameter (<4 mm) vascular constructs are urgently needed for patients requiring replacement of their peripheral vessels. However, successful development of constructs remains a significant challenge. In this study, we successfully developed small-diameter vascular constructs with high paten...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3411804?pdf=render |
id |
doaj-c98c76d096014df4968bf147b9614a35 |
---|---|
record_format |
Article |
spelling |
doaj-c98c76d096014df4968bf147b9614a352020-11-25T01:18:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4256910.1371/journal.pone.0042569Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor.Lei SongQiang ZhouPing DuanPing GuoDianwei LiYuan XuSongtao LiFei LuoZehua ZhangSmall-diameter (<4 mm) vascular constructs are urgently needed for patients requiring replacement of their peripheral vessels. However, successful development of constructs remains a significant challenge. In this study, we successfully developed small-diameter vascular constructs with high patency using our integrally designed computer-controlled bioreactor system. This computer-controlled bioreactor system can confer physiological mechanical stimuli and fluid flow similar to physiological stimuli to the cultured grafts. The medium circulating system optimizes the culture conditions by maintaining fixed concentration of O(2) and CO(2) in the medium flow and constant delivery of nutrients and waste metabolites, as well as eliminates the complicated replacement of culture medium in traditional vascular tissue engineering. Biochemical and mechanical assay of newly developed grafts confirm the feasibility of the bioreactor system for small-diameter vascular engineering. Furthermore, the computer-controlled bioreactor is superior for cultured cell proliferation compared with the traditional non-computer-controlled bioreactor. Specifically, our novel bioreactor system may be a potential alternative for tissue engineering of large-scale small-diameter vascular vessels for clinical use.http://europepmc.org/articles/PMC3411804?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Song Qiang Zhou Ping Duan Ping Guo Dianwei Li Yuan Xu Songtao Li Fei Luo Zehua Zhang |
spellingShingle |
Lei Song Qiang Zhou Ping Duan Ping Guo Dianwei Li Yuan Xu Songtao Li Fei Luo Zehua Zhang Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. PLoS ONE |
author_facet |
Lei Song Qiang Zhou Ping Duan Ping Guo Dianwei Li Yuan Xu Songtao Li Fei Luo Zehua Zhang |
author_sort |
Lei Song |
title |
Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
title_short |
Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
title_full |
Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
title_fullStr |
Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
title_full_unstemmed |
Successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
title_sort |
successful development of small diameter tissue-engineering vascular vessels by our novel integrally designed pulsatile perfusion-based bioreactor. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Small-diameter (<4 mm) vascular constructs are urgently needed for patients requiring replacement of their peripheral vessels. However, successful development of constructs remains a significant challenge. In this study, we successfully developed small-diameter vascular constructs with high patency using our integrally designed computer-controlled bioreactor system. This computer-controlled bioreactor system can confer physiological mechanical stimuli and fluid flow similar to physiological stimuli to the cultured grafts. The medium circulating system optimizes the culture conditions by maintaining fixed concentration of O(2) and CO(2) in the medium flow and constant delivery of nutrients and waste metabolites, as well as eliminates the complicated replacement of culture medium in traditional vascular tissue engineering. Biochemical and mechanical assay of newly developed grafts confirm the feasibility of the bioreactor system for small-diameter vascular engineering. Furthermore, the computer-controlled bioreactor is superior for cultured cell proliferation compared with the traditional non-computer-controlled bioreactor. Specifically, our novel bioreactor system may be a potential alternative for tissue engineering of large-scale small-diameter vascular vessels for clinical use. |
url |
http://europepmc.org/articles/PMC3411804?pdf=render |
work_keys_str_mv |
AT leisong successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT qiangzhou successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT pingduan successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT pingguo successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT dianweili successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT yuanxu successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT songtaoli successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT feiluo successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor AT zehuazhang successfuldevelopmentofsmalldiametertissueengineeringvascularvesselsbyournovelintegrallydesignedpulsatileperfusionbasedbioreactor |
_version_ |
1725142601695232000 |