Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance

Cas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.

Bibliographic Details
Main Authors:	Mohamed Fareh, Wei Zhao, Wenxin Hu, Joshua M. L. Casan, Amit Kumar, Jori Symons, Jennifer M. Zerbato, Danielle Fong, Ilia Voskoboinik, Paul G. Ekert, Rajeev Rudraraju, Damian F. J. Purcell, Sharon R. Lewin, Joseph A. Trapani
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-24577-9

id	doaj-c9a22d0a72d14aecb6991c4771cee7a6
record_format	Article
spelling	doaj-c9a22d0a72d14aecb6991c4771cee7a62021-07-18T11:43:07ZengNature Publishing GroupNature Communications2041-17232021-07-0112111610.1038/s41467-021-24577-9Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch toleranceMohamed Fareh0Wei Zhao1Wenxin Hu2Joshua M. L. Casan3Amit Kumar4Jori Symons5Jennifer M. Zerbato6Danielle Fong7Ilia Voskoboinik8Paul G. Ekert9Rajeev Rudraraju10Damian F. J. Purcell11Sharon R. Lewin12Joseph A. Trapani13Cancer Immunology Program, Peter MacCallum Cancer CentreThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityCancer Immunology Program, Peter MacCallum Cancer CentreCancer Immunology Program, Peter MacCallum Cancer CentreCancer Immunology Program, Peter MacCallum Cancer CentreThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityCancer Immunology Program, Peter MacCallum Cancer CentreCancer Immunology Program, Peter MacCallum Cancer CentreThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityDepartment of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityThe Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and ImmunityCancer Immunology Program, Peter MacCallum Cancer CentreCas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.https://doi.org/10.1038/s41467-021-24577-9
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani
spellingShingle	Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance Nature Communications
author_facet	Mohamed Fareh Wei Zhao Wenxin Hu Joshua M. L. Casan Amit Kumar Jori Symons Jennifer M. Zerbato Danielle Fong Ilia Voskoboinik Paul G. Ekert Rajeev Rudraraju Damian F. J. Purcell Sharon R. Lewin Joseph A. Trapani
author_sort	Mohamed Fareh
title	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_short	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_full	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_fullStr	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_full_unstemmed	Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
title_sort	reprogrammed crispr-cas13b suppresses sars-cov-2 replication and circumvents its mutational escape through mismatch tolerance
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-07-01
description	Cas13b can be harnessed to target and degrade RNA transcripts inside a cellular environment. Here the authors reprogram Cas13b to target SARSCoV-2 transcripts in infected mammalian cells and reveal its resilience to variants thanks to single mismatch tolerance.
url	https://doi.org/10.1038/s41467-021-24577-9
work_keys_str_mv	AT mohamedfareh reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT weizhao reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT wenxinhu reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT joshuamlcasan reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT amitkumar reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT jorisymons reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT jennifermzerbato reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT daniellefong reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT iliavoskoboinik reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT paulgekert reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT rajeevrudraraju reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT damianfjpurcell reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT sharonrlewin reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance AT josephatrapani reprogrammedcrisprcas13bsuppressessarscov2replicationandcircumventsitsmutationalescapethroughmismatchtolerance
_version_	1721295941206540288

Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance

Similar Items