Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease
Abstract Background Immunoglobulin A (IgA) and IgG are major components in human intestinal mucosal surface and sera, and IgA- or IgG-coated bacteria play a vital role in the intestinal homeostasis. However, the correlation of IgA, IgG and their coated bacteria with the clinical characteristics of i...
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doaj-c9c4ffc0eda443509d344c52cacde4512020-11-25T02:00:15ZengBMCJournal of Translational Medicine1479-58762018-12-0116111510.1186/s12967-018-1723-0Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel diseaseRitian Lin0Hongwei Chen1Weigang Shu2Mingming Sun3Leilei Fang4Yanhong Shi5Zhi Pang6Wei Wu7Zhanju Liu8Department of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, Luoyang Central Hospital Affiliated to Zhengzhou UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, Suzhou Municipal Hospital Affiliated to Nanjing Medical UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital of Tongji UniversityAbstract Background Immunoglobulin A (IgA) and IgG are major components in human intestinal mucosal surface and sera, and IgA- or IgG-coated bacteria play a vital role in the intestinal homeostasis. However, the correlation of IgA, IgG and their coated bacteria with the clinical characteristics of inflammatory bowel disease (IBD) has not been fully clarified. Methods The levels of soluble IgA and IgG in sera and feces were detected by ELISA, and the percentage of IgA- and IgG-coated bacteria in feces was analyzed by flow cytometry. Crohn’s disease activity index (CDAI) and Simple Endoscopic Score for Crohn’s disease (SES-CD) for Crohn’s disease (CD) or Mayo score and ulcerative colitis endoscopic index of severity (UCEIS) for ulcerative colitis (UC), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were used to evaluate the disease activity. Results 178 patients with CD, 75 patients with UC and 41 healthy donors were recruited in this study. We found that the concentrations of soluble IgA and IgG in feces of active IBD patients were significantly higher than those in healthy controls and that the levels of soluble IgA and IgG in feces from IBD patients were positively correlated with CRP, ESR, Mayo score, UCEIS, SES-CD, and CDAI, respectively. Moreover, we also observed that the percentage of IgA- and IgG-coated bacteria markedly increased in feces of IBD patients, especially in CD patients at the age of 17 to 40 years old, with terminal ileal lesions and perianal lesions, as well as from E2 UC patients, and was closely associated with disease activities. Conclusions The levels of soluble IgA and IgG and the percentage of IgA- and IgG-coated bacteria strikingly increase in feces of IBD patients and correlate with disease activity.http://link.springer.com/article/10.1186/s12967-018-1723-0Inflammatory bowel diseaseDisease activityImmunoglobulin AImmunoglobulin G |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ritian Lin Hongwei Chen Weigang Shu Mingming Sun Leilei Fang Yanhong Shi Zhi Pang Wei Wu Zhanju Liu |
spellingShingle |
Ritian Lin Hongwei Chen Weigang Shu Mingming Sun Leilei Fang Yanhong Shi Zhi Pang Wei Wu Zhanju Liu Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease Journal of Translational Medicine Inflammatory bowel disease Disease activity Immunoglobulin A Immunoglobulin G |
author_facet |
Ritian Lin Hongwei Chen Weigang Shu Mingming Sun Leilei Fang Yanhong Shi Zhi Pang Wei Wu Zhanju Liu |
author_sort |
Ritian Lin |
title |
Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease |
title_short |
Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease |
title_full |
Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease |
title_fullStr |
Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease |
title_full_unstemmed |
Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease |
title_sort |
clinical significance of soluble immunoglobulins a and g and their coated bacteria in feces of patients with inflammatory bowel disease |
publisher |
BMC |
series |
Journal of Translational Medicine |
issn |
1479-5876 |
publishDate |
2018-12-01 |
description |
Abstract Background Immunoglobulin A (IgA) and IgG are major components in human intestinal mucosal surface and sera, and IgA- or IgG-coated bacteria play a vital role in the intestinal homeostasis. However, the correlation of IgA, IgG and their coated bacteria with the clinical characteristics of inflammatory bowel disease (IBD) has not been fully clarified. Methods The levels of soluble IgA and IgG in sera and feces were detected by ELISA, and the percentage of IgA- and IgG-coated bacteria in feces was analyzed by flow cytometry. Crohn’s disease activity index (CDAI) and Simple Endoscopic Score for Crohn’s disease (SES-CD) for Crohn’s disease (CD) or Mayo score and ulcerative colitis endoscopic index of severity (UCEIS) for ulcerative colitis (UC), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were used to evaluate the disease activity. Results 178 patients with CD, 75 patients with UC and 41 healthy donors were recruited in this study. We found that the concentrations of soluble IgA and IgG in feces of active IBD patients were significantly higher than those in healthy controls and that the levels of soluble IgA and IgG in feces from IBD patients were positively correlated with CRP, ESR, Mayo score, UCEIS, SES-CD, and CDAI, respectively. Moreover, we also observed that the percentage of IgA- and IgG-coated bacteria markedly increased in feces of IBD patients, especially in CD patients at the age of 17 to 40 years old, with terminal ileal lesions and perianal lesions, as well as from E2 UC patients, and was closely associated with disease activities. Conclusions The levels of soluble IgA and IgG and the percentage of IgA- and IgG-coated bacteria strikingly increase in feces of IBD patients and correlate with disease activity. |
topic |
Inflammatory bowel disease Disease activity Immunoglobulin A Immunoglobulin G |
url |
http://link.springer.com/article/10.1186/s12967-018-1723-0 |
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