Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma
Background: Pancreatic ductal adenocarcinoma (PDAC), the most prevalent neoplastic lethal pancreatic disease, has a poor prognosis and an increasing incidence. The insulin-like growth factor-1 receptor (IGF-1R) signaling pathway is considered to be a contributing factor to the progression, metastasi...
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doaj-c9d485cfed1e4d58914938a106652cfa2021-08-26T13:36:52ZengMDPI AGCells2073-44092021-07-01101856185610.3390/cells10081856Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal AdenocarcinomaMaria Dobre0Vlad Herlea1Cătălina Vlăduţ2Mihai Ciocîrlan3Vasile Daniel Balaban4Gabriel Constantinescu5Mircea Diculescu6Elena Milanesi7Victor Babes National Institute of Pathology, 050096 Bucharest, RomaniaDepartment of Pathology, Fundeni Clinical Institute, 022328 Bucharest, RomaniaDepartment of Gastroenterology, “Prof Dr Agrippa Ionescu” Clinical Emergency Hospital, 011356 Bucharest, RomaniaDepartment of Gastroenterology, “Prof Dr Agrippa Ionescu” Clinical Emergency Hospital, 011356 Bucharest, RomaniaFaculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaDepartment of Gastroenterology, Clinical Emergency Hospital Bucharest, 014461 Bucharest, RomaniaFaculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaBackground: Pancreatic ductal adenocarcinoma (PDAC), the most prevalent neoplastic lethal pancreatic disease, has a poor prognosis and an increasing incidence. The insulin-like growth factor-1 receptor (IGF-1R) signaling pathway is considered to be a contributing factor to the progression, metastasis, and therapy resistance of PDAC. Currently available treatment options for PDAC are limited, but microRNAs (miRNAs) may represent a new therapeutic strategy for targeting genes involved in the IGF-1R signaling pathway. Method: We investigated the expression levels of 21 miRNAs involved in the IGF-1R signaling pathway in pancreatic tissue from 38 patients with PDAC and 11 controls (five patients with chronic pancreatitis and six patients with normal pancreatic tissue). Results: We found 19 differentially expressed miRNAs between the PDAC cases and the controls. In particular, miR-100-5p, miR-145-5p, miR-29c-3p, miR-9-5p, and miR-195-5p were exclusively downregulated in PDAC tissue but not in chronic pancreatitis or normal pancreatic tissues; both control types presented similar levels. We also identified miR-29a-3p, miR-29b-3p, and miR-7-5p as downregulated miRNAs in PDAC tissues as compared with normal tissues but not with pancreatitis tissues. Conclusions: We identified a panel of miRNAs that could represent putative therapeutic targets for the development of new miRNA-based therapies for PDAC.https://www.mdpi.com/2073-4409/10/8/1856pancreasadenocarcinomamicroRNAIGF-1R |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Dobre Vlad Herlea Cătălina Vlăduţ Mihai Ciocîrlan Vasile Daniel Balaban Gabriel Constantinescu Mircea Diculescu Elena Milanesi |
spellingShingle |
Maria Dobre Vlad Herlea Cătălina Vlăduţ Mihai Ciocîrlan Vasile Daniel Balaban Gabriel Constantinescu Mircea Diculescu Elena Milanesi Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma Cells pancreas adenocarcinoma microRNA IGF-1R |
author_facet |
Maria Dobre Vlad Herlea Cătălina Vlăduţ Mihai Ciocîrlan Vasile Daniel Balaban Gabriel Constantinescu Mircea Diculescu Elena Milanesi |
author_sort |
Maria Dobre |
title |
Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma |
title_short |
Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma |
title_full |
Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma |
title_fullStr |
Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma |
title_full_unstemmed |
Dysregulation of miRNAs Targeting the IGF-1R Pathway in Pancreatic Ductal Adenocarcinoma |
title_sort |
dysregulation of mirnas targeting the igf-1r pathway in pancreatic ductal adenocarcinoma |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-07-01 |
description |
Background: Pancreatic ductal adenocarcinoma (PDAC), the most prevalent neoplastic lethal pancreatic disease, has a poor prognosis and an increasing incidence. The insulin-like growth factor-1 receptor (IGF-1R) signaling pathway is considered to be a contributing factor to the progression, metastasis, and therapy resistance of PDAC. Currently available treatment options for PDAC are limited, but microRNAs (miRNAs) may represent a new therapeutic strategy for targeting genes involved in the IGF-1R signaling pathway. Method: We investigated the expression levels of 21 miRNAs involved in the IGF-1R signaling pathway in pancreatic tissue from 38 patients with PDAC and 11 controls (five patients with chronic pancreatitis and six patients with normal pancreatic tissue). Results: We found 19 differentially expressed miRNAs between the PDAC cases and the controls. In particular, miR-100-5p, miR-145-5p, miR-29c-3p, miR-9-5p, and miR-195-5p were exclusively downregulated in PDAC tissue but not in chronic pancreatitis or normal pancreatic tissues; both control types presented similar levels. We also identified miR-29a-3p, miR-29b-3p, and miR-7-5p as downregulated miRNAs in PDAC tissues as compared with normal tissues but not with pancreatitis tissues. Conclusions: We identified a panel of miRNAs that could represent putative therapeutic targets for the development of new miRNA-based therapies for PDAC. |
topic |
pancreas adenocarcinoma microRNA IGF-1R |
url |
https://www.mdpi.com/2073-4409/10/8/1856 |
work_keys_str_mv |
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