Preparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori

Preparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori

Show other versions (1)

"nBackground: Alkyl hydroperoxide reductase (AhpC) of Helicobacter pylori is considered as a diagnostic antigen. There&shy;fore, this antigen can be used to detect H. pylori infection by stool immunoassays such as ELISA. The aim of this study was to simplify the AhpC protein purific...

Full description

Bibliographic Details
Main Authors:	T Mohammadian, M Doosti, M Paknejad, F Siavoshi, S Massarrat
Format:	Article
Language:	English
Published:	Tehran University of Medical Sciences 2010-03-01
Series:	Iranian Journal of Public Health
Subjects:	Alkyl hydroperoxide reductase AhpC Electroelution Helicobacter pylori
Online Access:	http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15195.pdf&manuscript_id=15195

Similar Items

Preparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori
by: T Mohammadian, et al.
Published: (2010-03-01)

Construction and use of a prokaryotic expression system for <it>Helicobacter pylori</it> AhpC
by: Mehmood Khalid, et al.
Published: (2012-06-01)

Cellular iron metabolism and reductase systems in Escherichia coli and Shigella flexneri
by: Ma, Li, doctor of cellular and molecular biology
Published: (2012)

A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin from Clostridium septicum
by: Lorena Vázquez-Iglesias, et al.
Published: (2017-06-01)

Peroxiredoxin AhpC1 protects Pseudomonas aeruginosa against the inflammatory oxidative burst and confers virulence
by: Leonardo Silva Rocha, et al.
Published: (2021-10-01)

The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity
by: Basmarke-Wehelie, Rahma, et al.
Published: (2011)

Characteristics of 26 kDa Antigen of H. Pylori by Monoclonal Antibody
by: Hossein Ghahremani, et al.
Published: (2015-02-01)

Caracterização funcional e estrutural de peroxidases dependentes de tiól da bactéria fitopatogênica Xylella fastidiosa
by: Bruno Brasil Horta
Published: (2009)

Caracterização funcional e estrutural de peroxidases dependentes de tiól da bactéria fitopatogênica Xylella fastidiosa
by: Horta, Bruno Brasil
Published: (2009)

Distinct H2O2-Scavenging System in Yersinia pseudotuberculosis: KatG and AhpC Act Together to Scavenge Endogenous Hydrogen Peroxide
by: Fen Wan, et al.
Published: (2021-05-01)

Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus
by: Abd Razak, ZNR, et al.
Published: (2017)

Distinct Functions of Acyl/Alkyl Dihydroxyacetonephosphate Reductase in Peroxisomes and Endoplasmic Reticulum
by: Masanori Honsho, et al.
Published: (2020-09-01)

Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
by: Alice Di Pierro, et al.
Published: (2016-06-01)

Contribution of katG, ahpC and inhA mutations to the detection of isoniazid-resistant Mycobacterium tuberculosis isolates from Lebanon and Syria
by: F Dabboussi, et al.
Published: (2015-01-01)

Fatty acid hydroperoxides pathways in plants. A review.
by: M. L. Fauconnier, et al.
Published: (1997-02-01)

Deposit Formation of Deoxygenated JP-8 Fuel with Added Hydroperoxides
by: Kerr, Kristen Rita
Published: (2013)

Molecular Mechanisms of AhpC in Resistance to Oxidative Stress in Burkholderia thailandensis
by: Bing Zhang, et al.
Published: (2019-07-01)

Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant
by: Ning Rintiswati, et al.
Published: (2011-12-01)

NMR and Computational Studies as Analytical and High-Resolution Structural Tool for Complex Hydroperoxides and Diastereomeric <i>Endo</i>-Hydroperoxides of Fatty Acids in Solution-Exemplified by Methyl Linolenate
by: Raheel Ahmed, et al.
Published: (2020-10-01)

Hydroperoxides and potassium channels: a possible mechanism for vasodilation in septic shock.
by: Gotes Palazuelos, Jose
Published: (2013)

Hydroperoxides and potassium channels: a possible mechanism for vasodilation in septic shock.
by: Gotes Palazuelos, Jose
Published: (2013)

Investigation of Spectra and Ionization Constants of 8-Alkyl Pterins by Fluorimetry
by: Jeong Soon-Seog, et al.
Published: (1993-02-01)

DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN
by: V. V. Skarga, et al.
Published: (2019-02-01)

Pathophysiological potential of lipid hydroperoxide intermembrane translocation: Cholesterol hydroperoxide translocation as a special case
by: Albert W. Girotti, et al.
Published: (2021-10-01)

Oxidation of Organic Compounds with Peroxides Catalyzed by Polynuclear Metal Compounds
by: Georgiy B. Shul’pin, et al.
Published: (2021-01-01)

A Fluorescent Probe for the Specific Staining of Cysteine Containing Proteins and Thioredoxin Reductase in SDS-PAGE
by: Yuning Liu, et al.
Published: (2021-04-01)

Reaction mechanism of cumene hydroperoxide decomposition in cumene and evaluation of its reactivity hazards
by: Lu, Yuan
Published: (2010)

Producción y purificación de la proteína core del virus de la hepatitis C en el sistema de expresión del baculovirus para ensayos biológicos.
by: Ivonne Rubio, et al.
Published: (2005-03-01)

Quantification of Hydroperoxides by Gas Chromatography with Flame Ionisation Detection
by: Damjan Jan Pavlica, et al.
Published: (2021-09-01)

Lipid hydroperoxide generation, turnover, and effector action in biological systems
by: Albert W. Girotti
Published: (1998-08-01)

Purification of soybean lipoxygenase isoenzyme-1 and characterization of its inhibition by 13-hydroperoxides
by: M. L. Fauconnier, et al.
Published: (1996-08-01)

INFLUENCE OF TERT-BUTYL HYDROPEROXIDE AND NITROSOGLUTATHIONE ON Escherichia coli CELLS EXPRESSING LEGHEMOGLOBIN
by: Kosmachevskaya O.V.,, et al.
Published: (2007-06-01)

Isolation, Expression, and Characterization of a Hydroperoxide Lyase Gene from Cucumber
by: Xiao-Qing Shen, et al.
Published: (2013-11-01)

Synergistic Response of Rifampicin with Hydroperoxides on Mycobacterium: A Mechanistic Study
by: Yesha S. Patel, et al.
Published: (2017-10-01)

Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From Pseudomonas putida
by: Melanie Mindt, et al.
Published: (2019-09-01)

Microtubule Formation and Activities of Antioxidative Enzymes in PC12 Cells Exposed to Phosphatidylcholine Hydroperoxides
by: Yukako Yamanaka, et al.
Published: (2012-11-01)

The hydroperoxide moiety of aliphatic lipid hydroperoxides is not affected by hypochlorous acid
by: Zschaler, Josefin, et al.
Published: (2015)

Pigmented contact dermatitis in the axillae caused by hydroperoxides of limonene
by: Hui Li Kwong, MRCP (UK), et al.
Published: (2020-06-01)

Pseudomonas aeruginosa prioritizes detoxification of hydrogen peroxide over nitric oxide
by: Darshan M. Sivaloganathan, et al.
Published: (2021-03-01)

SHuffle, a novel <it>Escherichia coli</it> protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
by: Lobstein Julie, et al.
Published: (2012-05-01)