Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry
The normal growth pattern of female C57BL/6J mice, from 5 to 30 weeks of age, has been investigated in a longitudinal study. Weight, body surface area (BS), and body mass index (BMI) were evaluated in forty mice. Lean mass and fat mass, bone mineral content (BMC), and bone mineral density (BMD) were...
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doaj-c9d9c2abe73e4df5ba32dd9a065d586e2020-11-24T22:38:00ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/253067253067Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray AbsorptiometrySara Gargiulo0Matteo Gramanzini1Rosario Megna2Adelaide Greco3Sandra Albanese4Claudio Manfredi5Arturo Brunetti6Institute of Biostructures and Bioimages of National Council of Research of Naples, Via De Amicis 95, 80145 Naples, ItalyInstitute of Biostructures and Bioimages of National Council of Research of Naples, Via De Amicis 95, 80145 Naples, ItalyInstitute of Biostructures and Bioimages of National Council of Research of Naples, Via De Amicis 95, 80145 Naples, ItalyCEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, ItalyCEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, ItalyDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, Via Pansini 5, 80131 Naples, ItalyCEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, ItalyThe normal growth pattern of female C57BL/6J mice, from 5 to 30 weeks of age, has been investigated in a longitudinal study. Weight, body surface area (BS), and body mass index (BMI) were evaluated in forty mice. Lean mass and fat mass, bone mineral content (BMC), and bone mineral density (BMD) were monitored by dual energy X-ray absorptiometry (DEXA). Weight and BS increased linearly (16.15±0.64–27.64±1.42 g; 51.13±0.74–79.57±2.15 cm2, P<0.01), more markedly from 5 to 9 weeks of age (P<0.001). BMD showed a peak at 17 weeks (0.0548±0.0011 g/cm2* m, P<0.01). Lean mass showed an evident gain at 9 (15.8±0.8 g, P<0.001) and 25 weeks (20.5±0.3 g, P<0.01), like fat mass from 13 to 17 weeks (2.0±0.4–3.6±0.7 g, P<0.01). BMI and lean mass index (LMI) reached the highest value at 21 weeks (3.57±0.02–0.284±0.010 g/cm2, resp.), like fat mass index (FMI) at 17 weeks (0.057±0.009 g/cm2) (P<0.01). BMI, weight, and BS showed a moderate positive correlation (0.45–0.85) with lean mass from 5 to 21 weeks. Mixed linear models provided a good prediction for lean mass, fat mass, and BMD. This study may represent a baseline reference for a future comparison of wild-type C57BL/6J mice with models of altered growth.http://dx.doi.org/10.1155/2014/253067 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sara Gargiulo Matteo Gramanzini Rosario Megna Adelaide Greco Sandra Albanese Claudio Manfredi Arturo Brunetti |
spellingShingle |
Sara Gargiulo Matteo Gramanzini Rosario Megna Adelaide Greco Sandra Albanese Claudio Manfredi Arturo Brunetti Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry BioMed Research International |
author_facet |
Sara Gargiulo Matteo Gramanzini Rosario Megna Adelaide Greco Sandra Albanese Claudio Manfredi Arturo Brunetti |
author_sort |
Sara Gargiulo |
title |
Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry |
title_short |
Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry |
title_full |
Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry |
title_fullStr |
Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry |
title_full_unstemmed |
Evaluation of Growth Patterns and Body Composition in C57Bl/6J Mice Using Dual Energy X-Ray Absorptiometry |
title_sort |
evaluation of growth patterns and body composition in c57bl/6j mice using dual energy x-ray absorptiometry |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
The normal growth pattern of female C57BL/6J mice, from 5 to 30 weeks of age, has been investigated in a longitudinal study. Weight, body surface area (BS), and body mass index (BMI) were evaluated in forty mice. Lean mass and fat mass, bone mineral content (BMC), and bone mineral density (BMD) were monitored by dual energy X-ray absorptiometry (DEXA). Weight and BS increased linearly (16.15±0.64–27.64±1.42 g; 51.13±0.74–79.57±2.15 cm2, P<0.01), more markedly from 5 to 9 weeks of age (P<0.001). BMD showed a peak at 17 weeks (0.0548±0.0011 g/cm2* m, P<0.01). Lean mass showed an evident gain at 9 (15.8±0.8 g, P<0.001) and 25 weeks (20.5±0.3 g, P<0.01), like fat mass from 13 to 17 weeks (2.0±0.4–3.6±0.7 g, P<0.01). BMI and lean mass index (LMI) reached the highest value at 21 weeks (3.57±0.02–0.284±0.010 g/cm2, resp.), like fat mass index (FMI) at 17 weeks (0.057±0.009 g/cm2) (P<0.01). BMI, weight, and BS showed a moderate positive correlation (0.45–0.85) with lean mass from 5 to 21 weeks. Mixed linear models provided a good prediction for lean mass, fat mass, and BMD. This study may represent a baseline reference for a future comparison of wild-type C57BL/6J mice with models of altered growth. |
url |
http://dx.doi.org/10.1155/2014/253067 |
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