Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. We studied the secretome profile of gestational tissue-derived MSCs and t...
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doaj-c9e1cbbf3fb44af49f389447d9f7a4902020-11-25T01:11:48ZengHindawi LimitedStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/25143262514326Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem CellsWitchayaporn Kamprom0Pakpoom Kheolamai1Yaowalak U-Pratya2Aungkura Supokawej3Methichit Wattanapanitch4Chuti Laowtammathron5Sittiruk Roytrakul6Surapol Issaragrisil7Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok 73170, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandProteomics Research Laboratory, Genome Institute, National Science and Technology Development Agency, Pathumthani 12120, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandTherapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. MSCs derived from placental tissues (PL-MSCs) significantly enhanced EPC migration while BM-MSCs, which are the standard source of MSCs for various clinical applications, did not. By using protein fractionation and mass spectrometry analysis, we identified several novel candidates for EPC migration enhancing factor in PL-MSCs secretome that could be used to enhance neovascularization in the injured/ischemic tissues. We recommend that the strategy developed in our study could be used to systematically identify therapeutically useful molecules in the secretomes of other MSC sources for the clinical applications.http://dx.doi.org/10.1155/2016/2514326 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Witchayaporn Kamprom Pakpoom Kheolamai Yaowalak U-Pratya Aungkura Supokawej Methichit Wattanapanitch Chuti Laowtammathron Sittiruk Roytrakul Surapol Issaragrisil |
spellingShingle |
Witchayaporn Kamprom Pakpoom Kheolamai Yaowalak U-Pratya Aungkura Supokawej Methichit Wattanapanitch Chuti Laowtammathron Sittiruk Roytrakul Surapol Issaragrisil Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells Stem Cells International |
author_facet |
Witchayaporn Kamprom Pakpoom Kheolamai Yaowalak U-Pratya Aungkura Supokawej Methichit Wattanapanitch Chuti Laowtammathron Sittiruk Roytrakul Surapol Issaragrisil |
author_sort |
Witchayaporn Kamprom |
title |
Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells |
title_short |
Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells |
title_full |
Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells |
title_fullStr |
Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells |
title_full_unstemmed |
Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells |
title_sort |
endothelial progenitor cell migration-enhancing factors in the secretome of placental-derived mesenchymal stem cells |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2016-01-01 |
description |
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. MSCs derived from placental tissues (PL-MSCs) significantly enhanced EPC migration while BM-MSCs, which are the standard source of MSCs for various clinical applications, did not. By using protein fractionation and mass spectrometry analysis, we identified several novel candidates for EPC migration enhancing factor in PL-MSCs secretome that could be used to enhance neovascularization in the injured/ischemic tissues. We recommend that the strategy developed in our study could be used to systematically identify therapeutically useful molecules in the secretomes of other MSC sources for the clinical applications. |
url |
http://dx.doi.org/10.1155/2016/2514326 |
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