Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo
<p>This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced <em>in vivo</em>. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was performed to verify th...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universidade Estadual de Londrina
2015-12-01
|
Series: | Semina: Ciências Agrárias |
Subjects: | |
Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19932 |
id |
doaj-c9fca17124614bb3877d23ea4287f4e1 |
---|---|
record_format |
Article |
spelling |
doaj-c9fca17124614bb3877d23ea4287f4e12020-11-24T21:28:14ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592015-12-01366Supl24257426810.5433/1679-0359.2015v36n6Sup2p425712745Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivoAlane Pains Oliveira do Monte0João Bosco Loiola Filho1Thais Thatiane dos Santos Souza2Mayara de Souza Miranda3Lívia Correia Magalhães4Celso Henrique Souza Costa Barros5Ana Arlete de Amorim Silva6Adriano Oliveira Santos7Aionne de Souza Leite Guimarães8Jonathan Maia da Silva Costa9Pedro Humberto Félix de Sousa10Daniel Maia Nogueira11Mabel Freitas Cordeiro12Edilson Soares Lopes Júnior13Universidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São FranciscoUniversidade do Estado da BahiaEmpresa Brasileira de Pesquisa AgropecuáriaUniversidade Federal do Vale do São FranciscoUniversidade Federal do Vale do São Francisco<p>This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced <em>in vivo</em>. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was performed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from Sucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.</p>http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19932CryopreservationEmbryoEweMultiple ovulationSugar. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alane Pains Oliveira do Monte João Bosco Loiola Filho Thais Thatiane dos Santos Souza Mayara de Souza Miranda Lívia Correia Magalhães Celso Henrique Souza Costa Barros Ana Arlete de Amorim Silva Adriano Oliveira Santos Aionne de Souza Leite Guimarães Jonathan Maia da Silva Costa Pedro Humberto Félix de Sousa Daniel Maia Nogueira Mabel Freitas Cordeiro Edilson Soares Lopes Júnior |
spellingShingle |
Alane Pains Oliveira do Monte João Bosco Loiola Filho Thais Thatiane dos Santos Souza Mayara de Souza Miranda Lívia Correia Magalhães Celso Henrique Souza Costa Barros Ana Arlete de Amorim Silva Adriano Oliveira Santos Aionne de Souza Leite Guimarães Jonathan Maia da Silva Costa Pedro Humberto Félix de Sousa Daniel Maia Nogueira Mabel Freitas Cordeiro Edilson Soares Lopes Júnior Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo Semina: Ciências Agrárias Cryopreservation Embryo Ewe Multiple ovulation Sugar. |
author_facet |
Alane Pains Oliveira do Monte João Bosco Loiola Filho Thais Thatiane dos Santos Souza Mayara de Souza Miranda Lívia Correia Magalhães Celso Henrique Souza Costa Barros Ana Arlete de Amorim Silva Adriano Oliveira Santos Aionne de Souza Leite Guimarães Jonathan Maia da Silva Costa Pedro Humberto Félix de Sousa Daniel Maia Nogueira Mabel Freitas Cordeiro Edilson Soares Lopes Júnior |
author_sort |
Alane Pains Oliveira do Monte |
title |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo |
title_short |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo |
title_full |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo |
title_fullStr |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo |
title_full_unstemmed |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo |
title_sort |
addition of sucrose on cryoprotectant solution of vitrification enhances the quality of dorper sheep embryos produced in vivo |
publisher |
Universidade Estadual de Londrina |
series |
Semina: Ciências Agrárias |
issn |
1676-546X 1679-0359 |
publishDate |
2015-12-01 |
description |
<p>This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced <em>in vivo</em>. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was performed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from Sucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.</p> |
topic |
Cryopreservation Embryo Ewe Multiple ovulation Sugar. |
url |
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/19932 |
work_keys_str_mv |
AT alanepainsoliveiradomonte additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT joaoboscoloiolafilho additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT thaisthatianedossantossouza additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT mayaradesouzamiranda additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT liviacorreiamagalhaes additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT celsohenriquesouzacostabarros additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT anaarletedeamorimsilva additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT adrianooliveirasantos additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT aionnedesouzaleiteguimaraes additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT jonathanmaiadasilvacosta additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT pedrohumbertofelixdesousa additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT danielmaianogueira additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT mabelfreitascordeiro additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo AT edilsonsoareslopesjunior additionofsucroseoncryoprotectantsolutionofvitrificationenhancesthequalityofdorpersheepembryosproducedinvivo |
_version_ |
1725971504930226176 |