RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
RAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expre...
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doaj-ca09e42973f44451a5f9fc33bf69cb972020-11-24T21:41:05ZengBioscientificaEndocrine Connections2049-36142049-36142017-10-016870070710.1530/EC-17-0209RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinomaWei Li10Qing Huang1Danyang Sun2Guizhi Zhang3Jian Tan4Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaCollage of Tourism and Service Management, Nankai University, Tianjin, People’s Republic of ChinaDepartment of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaRAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expression in PTC patients was examined using immunohistochemistry. The expression levels of RDM1 mRNA in thyroid cancer cells were measured by quantitative real-time PCR (qRT-PCR). Lentivirus-mediated small interfering RNAs (siRNAs) were used to knock down the RDM1 expression in the K1 and TPC1 cells. Then, changes in the RDM1 target gene expression were determined by qRT-PCR and Western blot. Cell proliferation was examined by a high content screening assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis and MTT analysis. We showed that the RDM1 expression was higher in PTC tissue compared to pericarcinous tissue. RDM1 mRNA was found to be expressed by qRT-PCR. Using a lentivirus-based RNA interference (RNAi) approach, the RDM1 expression was significantly inhibited. The inhibition of RDM1 expression by RNAi significantly impaired cell proliferation, increased apoptosis and arrested cells in the G2/M phase. These data showed that RDM1 was highly expressed in PTC tissue and thyroid cancer cell lines. Moreover, RDM1 may play an important role in cell proliferation, cell cycle distribution and apoptosis of human PTC cells.http://www.endocrineconnections.com/content/6/8/700.fullRDM1thyroid cancerpapillary thyroid carcinomasiRNAlentivirus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Li1 Qing Huang Danyang Sun Guizhi Zhang Jian Tan |
spellingShingle |
Wei Li1 Qing Huang Danyang Sun Guizhi Zhang Jian Tan RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma Endocrine Connections RDM1 thyroid cancer papillary thyroid carcinoma siRNA lentivirus |
author_facet |
Wei Li1 Qing Huang Danyang Sun Guizhi Zhang Jian Tan |
author_sort |
Wei Li1 |
title |
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
title_short |
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
title_full |
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
title_fullStr |
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
title_full_unstemmed |
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
title_sort |
rdm1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma |
publisher |
Bioscientifica |
series |
Endocrine Connections |
issn |
2049-3614 2049-3614 |
publishDate |
2017-10-01 |
description |
RAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expression in PTC patients was examined using immunohistochemistry. The expression levels of RDM1 mRNA in thyroid cancer cells were measured by quantitative real-time PCR (qRT-PCR). Lentivirus-mediated small interfering RNAs (siRNAs) were used to knock down the RDM1 expression in the K1 and TPC1 cells. Then, changes in the RDM1 target gene expression were determined by qRT-PCR and Western blot. Cell proliferation was examined by a high content screening assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis and MTT analysis. We showed that the RDM1 expression was higher in PTC tissue compared to pericarcinous tissue. RDM1 mRNA was found to be expressed by qRT-PCR. Using a lentivirus-based RNA interference (RNAi) approach, the RDM1 expression was significantly inhibited. The inhibition of RDM1 expression by RNAi significantly impaired cell proliferation, increased apoptosis and arrested cells in the G2/M phase. These data showed that RDM1 was highly expressed in PTC tissue and thyroid cancer cell lines. Moreover, RDM1 may play an important role in cell proliferation, cell cycle distribution and apoptosis of human PTC cells. |
topic |
RDM1 thyroid cancer papillary thyroid carcinoma siRNA lentivirus |
url |
http://www.endocrineconnections.com/content/6/8/700.full |
work_keys_str_mv |
AT weili1 rdm1geneoverexpressionrepresentsatherapeutictargetinpapillarythyroidcarcinoma AT qinghuang rdm1geneoverexpressionrepresentsatherapeutictargetinpapillarythyroidcarcinoma AT danyangsun rdm1geneoverexpressionrepresentsatherapeutictargetinpapillarythyroidcarcinoma AT guizhizhang rdm1geneoverexpressionrepresentsatherapeutictargetinpapillarythyroidcarcinoma AT jiantan rdm1geneoverexpressionrepresentsatherapeutictargetinpapillarythyroidcarcinoma |
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