Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1
PolyADP-ribosylation (PARylation) is a posttranslational modification that is involved in the various cellular functions including DNA repair, genomic stability, and transcriptional regulation. PARylation is catalyzed by the poly(ADP-ribose) polymerase (PARP) family proteins, which mainly recognize...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Biophysical Society of Japan
2019-02-01
|
Series: | Biophysics and Physicobiology |
Subjects: | |
Online Access: | https://doi.org/10.2142/biophysico.16.0_59 |
id |
doaj-ca1b3311633549819dbecafe9e1538b5 |
---|---|
record_format |
Article |
spelling |
doaj-ca1b3311633549819dbecafe9e1538b52020-11-25T03:50:15ZengThe Biophysical Society of JapanBiophysics and Physicobiology2189-47792019-02-011610.2142/biophysico.16.0_59Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1Kenichi Kouyama0Kouta Mayanagi1Setsu Nakae2Yoshisuke Nishi3Masanao Miwa4Tsuyoshi Shirai5Nagahama Institute of BioScience and Technology, Nagahama, Shiga 526-0829, JapanMedical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanNagahama Institute of BioScience and Technology, Nagahama, Shiga 526-0829, JapanNagahama Institute of BioScience and Technology, Nagahama, Shiga 526-0829, JapanNagahama Institute of BioScience and Technology, Nagahama, Shiga 526-0829, JapanNagahama Institute of BioScience and Technology, Nagahama, Shiga 526-0829, JapanPolyADP-ribosylation (PARylation) is a posttranslational modification that is involved in the various cellular functions including DNA repair, genomic stability, and transcriptional regulation. PARylation is catalyzed by the poly(ADP-ribose) polymerase (PARP) family proteins, which mainly recognize damaged DNA and initiate repair processes. PARP inhibitors are expected to be novel anticancer drugs for breast and ovarian cancers having mutation in BRCA tumor suppressor genes. However the structure of intact (full-length) PARP is not yet known. We have produced and purified the full-length human PARP1 (h-PARP1), which is the major family member of PARPs, and analyzed it with single particle electron microscopy. The electron microscopic images and the reconstructed 3D density map revealed a dimeric configuration of the h-PARP1, in which two ring-shaped subunits are associated with two-fold symmetry. Although the PARP1 is hypothesized to form a dimer on damaged DNA, the quaternary structure of this protein is still controversial. The present result would provide the first structural evidence of the dimeric structure of PARP1.https://doi.org/10.2142/biophysico.16.0_59parp1dna repairelectron microscopystructural biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kenichi Kouyama Kouta Mayanagi Setsu Nakae Yoshisuke Nishi Masanao Miwa Tsuyoshi Shirai |
spellingShingle |
Kenichi Kouyama Kouta Mayanagi Setsu Nakae Yoshisuke Nishi Masanao Miwa Tsuyoshi Shirai Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 Biophysics and Physicobiology parp1 dna repair electron microscopy structural biology |
author_facet |
Kenichi Kouyama Kouta Mayanagi Setsu Nakae Yoshisuke Nishi Masanao Miwa Tsuyoshi Shirai |
author_sort |
Kenichi Kouyama |
title |
Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 |
title_short |
Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 |
title_full |
Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 |
title_fullStr |
Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 |
title_full_unstemmed |
Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1 |
title_sort |
single-particle analysis of full-length human poly(adp-ribose) polymerase 1 |
publisher |
The Biophysical Society of Japan |
series |
Biophysics and Physicobiology |
issn |
2189-4779 |
publishDate |
2019-02-01 |
description |
PolyADP-ribosylation (PARylation) is a posttranslational modification that is involved in the various cellular functions including DNA repair, genomic stability, and transcriptional regulation. PARylation is catalyzed by the poly(ADP-ribose) polymerase (PARP) family proteins, which mainly recognize damaged DNA and initiate repair processes. PARP inhibitors are expected to be novel anticancer drugs for breast and ovarian cancers having mutation in BRCA tumor suppressor genes. However the structure of intact (full-length) PARP is not yet known. We have produced and purified the full-length human PARP1 (h-PARP1), which is the major family member of PARPs, and analyzed it with single particle electron microscopy. The electron microscopic images and the reconstructed 3D density map revealed a dimeric configuration of the h-PARP1, in which two ring-shaped subunits are associated with two-fold symmetry. Although the PARP1 is hypothesized to form a dimer on damaged DNA, the quaternary structure of this protein is still controversial. The present result would provide the first structural evidence of the dimeric structure of PARP1. |
topic |
parp1 dna repair electron microscopy structural biology |
url |
https://doi.org/10.2142/biophysico.16.0_59 |
work_keys_str_mv |
AT kenichikouyama singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 AT koutamayanagi singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 AT setsunakae singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 AT yoshisukenishi singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 AT masanaomiwa singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 AT tsuyoshishirai singleparticleanalysisoffulllengthhumanpolyadpribosepolymerase1 |
_version_ |
1724491522779381760 |