Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis
A simple, sensitive, and specific capillary electrophoretic method based on 1-phenyl-3-methyl-5-pyrazolon (PMP) derivatization has been developed for simultaneous separation and determination of 11 aldoses (maltose, xylose, arabinose, ribose, glucose, rhamnose, fucose, galactose, mannose, glucuronic...
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Online Access: | http://dx.doi.org/10.1080/19476337.2017.1308970 |
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doaj-ca70d16467414fb28bcdf7063b9956272020-11-25T00:57:27ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452017-10-0115453153710.1080/19476337.2017.13089701308970Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresisYalong Lu0Yuanyuan Hu1Tong Wang2Xingbin Yang3Yan Zhao4Shaanxi Normal UniversityChongqing University of EducationFourth Military Medical UniversityShaanxi Normal UniversityFourth Military Medical UniversityA simple, sensitive, and specific capillary electrophoretic method based on 1-phenyl-3-methyl-5-pyrazolon (PMP) derivatization has been developed for simultaneous separation and determination of 11 aldoses (maltose, xylose, arabinose, ribose, glucose, rhamnose, fucose, galactose, mannose, glucuronic acid, and galacturonic acid). The separation of PMP-labeled maltose and monosaccharides was carried out under the selected optimum conditions with pH 11.0, 200 mM borate buffer containing 4% methanol at voltage 20 kV, and capillary temperature 25°C, and the 11 PMP aldoses could be perfectly separated from each other within 41 min. Our experiment results demonstrated that the molar ratios of carbohydrates in 10 kinds of honey were greatly different, and the carbohydrates’ constitutions varied greatly between commodity honey and natural honey. This proposed zone electrophoresis method provides an accurate and economic alternative for monosaccharide and maltose analysis to identify honeys, and can also be applied to routine detection of reducing carbohydrates in real-life samples.http://dx.doi.org/10.1080/19476337.2017.1308970Honeypolysaccharidesmonosaccharidemaltosecapillary zone electrophoresis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yalong Lu Yuanyuan Hu Tong Wang Xingbin Yang Yan Zhao |
spellingShingle |
Yalong Lu Yuanyuan Hu Tong Wang Xingbin Yang Yan Zhao Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis CyTA - Journal of Food Honey polysaccharides monosaccharide maltose capillary zone electrophoresis |
author_facet |
Yalong Lu Yuanyuan Hu Tong Wang Xingbin Yang Yan Zhao |
author_sort |
Yalong Lu |
title |
Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
title_short |
Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
title_full |
Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
title_fullStr |
Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
title_full_unstemmed |
Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
title_sort |
rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis |
publisher |
Taylor & Francis Group |
series |
CyTA - Journal of Food |
issn |
1947-6337 1947-6345 |
publishDate |
2017-10-01 |
description |
A simple, sensitive, and specific capillary electrophoretic method based on 1-phenyl-3-methyl-5-pyrazolon (PMP) derivatization has been developed for simultaneous separation and determination of 11 aldoses (maltose, xylose, arabinose, ribose, glucose, rhamnose, fucose, galactose, mannose, glucuronic acid, and galacturonic acid). The separation of PMP-labeled maltose and monosaccharides was carried out under the selected optimum conditions with pH 11.0, 200 mM borate buffer containing 4% methanol at voltage 20 kV, and capillary temperature 25°C, and the 11 PMP aldoses could be perfectly separated from each other within 41 min. Our experiment results demonstrated that the molar ratios of carbohydrates in 10 kinds of honey were greatly different, and the carbohydrates’ constitutions varied greatly between commodity honey and natural honey. This proposed zone electrophoresis method provides an accurate and economic alternative for monosaccharide and maltose analysis to identify honeys, and can also be applied to routine detection of reducing carbohydrates in real-life samples. |
topic |
Honey polysaccharides monosaccharide maltose capillary zone electrophoresis |
url |
http://dx.doi.org/10.1080/19476337.2017.1308970 |
work_keys_str_mv |
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