Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction

Background This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using...

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Main Authors: Chul-Hoon Choi, Ho-Sueb Song
Format: Article
Language:English
Published: E-Tree Publishing 2019-08-01
Series:Journal of Acupuncture Research
Subjects:
Online Access:http://www.e-jar.org/upload/pdf/jar-2019-00073.pdf
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spelling doaj-ca7f1dd077c94f2fbfbb20ba19051da22020-11-25T01:40:07ZengE-Tree PublishingJournal of Acupuncture Research2586-288X2586-28982019-08-0136314014610.13045/jar.2019.000732451Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral InfarctionChul-Hoon Choi0Ho-Sueb Song1 Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, Korea Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, KoreaBackground This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using triphenyltetrazolium chloride (TTC) staining, neurological deficit score, NO, ROS, and GSH/GSSG assays, qPCR, Western blot, and cell viability. Results Cerebral infarction caused by middle cerebral artery occlusion as observed by TTC staining, showed SV inhibited cell death, reducing the number of brain cells injured due to infarction. SV treatment for cerebral infarction showed a significant decrease in abnormal behavior, as determined by the neurological deficit score. The oxidation and inflammation of the cells that had cerebral infarction caused by middle cerebral artery occlusion (NO assay, ROS, GSH/GSSG assay, and qPCR), showed significant protection by SV. Western blot of brain infarction cells showed the expression of iNOS, COX-2, p-IkB-α, P38, p-JNK, p-ERK to be lower in the SV group. In addition, the expression of IkB increased. BV2 cells were viable when treated with SV at 20 μg/mL or less. Western blot of BV2 cells, treated with 0.625, 1.5, 2.5 μg/mL of SV, showed a significant decrease in the expression of p-IkB-α, p-JNK, iNOS, and COX-2 on BV2 cells induced by LPS. Conclusions SV showed anti-inflammatory and anti-oxidant effects against cerebral infarction and inflammation.http://www.e-jar.org/upload/pdf/jar-2019-00073.pdfcerebral infarctioninflammationmiddle cerebral artery occlusionpharmacopuncturesnake venom
collection DOAJ
language English
format Article
sources DOAJ
author Chul-Hoon Choi
Ho-Sueb Song
spellingShingle Chul-Hoon Choi
Ho-Sueb Song
Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
Journal of Acupuncture Research
cerebral infarction
inflammation
middle cerebral artery occlusion
pharmacopuncture
snake venom
author_facet Chul-Hoon Choi
Ho-Sueb Song
author_sort Chul-Hoon Choi
title Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
title_short Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
title_full Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
title_fullStr Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
title_full_unstemmed Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
title_sort effects of snake venom pharmacopuncture on a mouse model of cerebral infarction
publisher E-Tree Publishing
series Journal of Acupuncture Research
issn 2586-288X
2586-2898
publishDate 2019-08-01
description Background This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using triphenyltetrazolium chloride (TTC) staining, neurological deficit score, NO, ROS, and GSH/GSSG assays, qPCR, Western blot, and cell viability. Results Cerebral infarction caused by middle cerebral artery occlusion as observed by TTC staining, showed SV inhibited cell death, reducing the number of brain cells injured due to infarction. SV treatment for cerebral infarction showed a significant decrease in abnormal behavior, as determined by the neurological deficit score. The oxidation and inflammation of the cells that had cerebral infarction caused by middle cerebral artery occlusion (NO assay, ROS, GSH/GSSG assay, and qPCR), showed significant protection by SV. Western blot of brain infarction cells showed the expression of iNOS, COX-2, p-IkB-α, P38, p-JNK, p-ERK to be lower in the SV group. In addition, the expression of IkB increased. BV2 cells were viable when treated with SV at 20 μg/mL or less. Western blot of BV2 cells, treated with 0.625, 1.5, 2.5 μg/mL of SV, showed a significant decrease in the expression of p-IkB-α, p-JNK, iNOS, and COX-2 on BV2 cells induced by LPS. Conclusions SV showed anti-inflammatory and anti-oxidant effects against cerebral infarction and inflammation.
topic cerebral infarction
inflammation
middle cerebral artery occlusion
pharmacopuncture
snake venom
url http://www.e-jar.org/upload/pdf/jar-2019-00073.pdf
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