Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain

• Main Authors: Asmaa Abdella, Fernando Segato, Mark R. Wilkins
• Format: Article
• Language: English
• Published: Elsevier 2020-06-01
• Series: Biotechnology Reports
• Subjects: Xylanase; Enzyme; Fed batch; Repeated batch; Optimization
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215017X20300576
• ISSN: 2215-017X

The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.