Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain

The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with prod...

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Main Authors:	Asmaa Abdella, Fernando Segato, Mark R. Wilkins
Format:	Article
Language:	English
Published:	Elsevier 2020-06-01
Series:	Biotechnology Reports
Subjects:	Xylanase Enzyme Fed batch Repeated batch Optimization
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215017X20300576

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spelling	doaj-ca82c9d55aff445890de9c8bf3dc7a572020-11-25T03:41:25ZengElsevierBiotechnology Reports2215-017X2020-06-0126e00457Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strainAsmaa Abdella0Fernando Segato1Mark R. Wilkins2Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, PO Box 79, Sadat City, 22857 Egypt; Department of Biological Systems Engineering, University of Nebraska-Lincoln, 3605 Fair Street, Lincoln, NE, 68583-0726 USA; Industrial Agricultural Products Center, University of Nebraska-Lincoln, 3605 Fair Street, Lincoln, NE, 68583-0730 USASynthetic and Molecular Biology Laboratory, Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Estrada Municipal do Campinho, s/n, Lorena, SP, BrazilDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, 3605 Fair Street, Lincoln, NE, 68583-0726 USA; Industrial Agricultural Products Center, University of Nebraska-Lincoln, 3605 Fair Street, Lincoln, NE, 68583-0730 USA; Department of Food Science and Technology, 1901 N 21stSt. University of Nebraska-Lincoln, Lincoln, NE, 68588-6205 USA; Corresponding author at: University of Nebraska-Lincoln, 211 Chase Hall, PO Box 830726, Lincoln, NE, 68583-0726 USA.The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.http://www.sciencedirect.com/science/article/pii/S2215017X20300576XylanaseEnzymeFed batchRepeated batchOptimization
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Asmaa Abdella Fernando Segato Mark R. Wilkins
spellingShingle	Asmaa Abdella Fernando Segato Mark R. Wilkins Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain Biotechnology Reports Xylanase Enzyme Fed batch Repeated batch Optimization
author_facet	Asmaa Abdella Fernando Segato Mark R. Wilkins
author_sort	Asmaa Abdella
title	Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
title_short	Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
title_full	Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
title_fullStr	Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
title_full_unstemmed	Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
title_sort	optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant aspergillus nidulans strain
publisher	Elsevier
series	Biotechnology Reports
issn	2215-017X
publishDate	2020-06-01
description	The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
topic	Xylanase Enzyme Fed batch Repeated batch Optimization
url	http://www.sciencedirect.com/science/article/pii/S2215017X20300576
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Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain

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