RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods

RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods

Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR). However, RT-qPCR can only be perf...

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Main Authors: Kasturi Selvam, Mohamad Ahmad Najib, Muhammad Fazli Khalid, Suharni Mohamad, Fahreddin Palaz, Mehmet Ozsoz, Ismail Aziah
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Diagnostics
Subjects:
COVID-19
SARS-CoV-2
RT-LAMP
CRISPR
Cas12
Cas13
Online Access:https://www.mdpi.com/2075-4418/11/9/1646
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spelling doaj-ca8367ea158645c3b876e4eb53a29aad2021-09-25T23:59:16ZengMDPI AGDiagnostics2075-44182021-09-01111646164610.3390/diagnostics11091646RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection MethodsKasturi Selvam0Mohamad Ahmad Najib1Muhammad Fazli Khalid2Suharni Mohamad3Fahreddin Palaz4Mehmet Ozsoz5Ismail Aziah6Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaSchool of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaFaculty of Medicine, Hacettepe University, Ankara 06100, TurkeyInstitute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, MalaysiaCoronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR). However, RT-qPCR can only be performed in centralized laboratories due to the requirement for advanced laboratory equipment and qualified workers. In the last decade, clustered regularly interspaced short palindromic repeats (CRISPR) technology has shown considerable promise in the development of rapid, highly sensitive, and specific molecular diagnostic methods that do not require complicated instrumentation. During the current COVID-19 pandemic, there has been growing interest in using CRISPR-based diagnostic techniques to develop rapid and accurate assays for detecting SARS-CoV-2. In this work, we review and summarize reverse-transcription loop-mediated isothermal amplification (RT-LAMP) CRISPR-based diagnostic techniques for detecting SARS-CoV-2.https://www.mdpi.com/2075-4418/11/9/1646COVID-19SARS-CoV-2RT-LAMPCRISPRCas12Cas13
collection DOAJ
language English
format Article
sources DOAJ
author Kasturi Selvam
Mohamad Ahmad Najib
Muhammad Fazli Khalid
Suharni Mohamad
Fahreddin Palaz
Mehmet Ozsoz
Ismail Aziah
spellingShingle Kasturi Selvam
Mohamad Ahmad Najib
Muhammad Fazli Khalid
Suharni Mohamad
Fahreddin Palaz
Mehmet Ozsoz
Ismail Aziah
RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
Diagnostics
COVID-19
SARS-CoV-2
RT-LAMP
CRISPR
Cas12
Cas13
author_facet Kasturi Selvam
Mohamad Ahmad Najib
Muhammad Fazli Khalid
Suharni Mohamad
Fahreddin Palaz
Mehmet Ozsoz
Ismail Aziah
author_sort Kasturi Selvam
title RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
title_short RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
title_full RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
title_fullStr RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
title_full_unstemmed RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods
title_sort rt-lamp crispr-cas12/13-based sars-cov-2 detection methods
publisher MDPI AG
series Diagnostics
issn 2075-4418
publishDate 2021-09-01
description Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription–quantitative polymerase chain reaction (RT-qPCR). However, RT-qPCR can only be performed in centralized laboratories due to the requirement for advanced laboratory equipment and qualified workers. In the last decade, clustered regularly interspaced short palindromic repeats (CRISPR) technology has shown considerable promise in the development of rapid, highly sensitive, and specific molecular diagnostic methods that do not require complicated instrumentation. During the current COVID-19 pandemic, there has been growing interest in using CRISPR-based diagnostic techniques to develop rapid and accurate assays for detecting SARS-CoV-2. In this work, we review and summarize reverse-transcription loop-mediated isothermal amplification (RT-LAMP) CRISPR-based diagnostic techniques for detecting SARS-CoV-2.
topic COVID-19
SARS-CoV-2
RT-LAMP
CRISPR
Cas12
Cas13
url https://www.mdpi.com/2075-4418/11/9/1646
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