Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella...
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Frontiers Media S.A.
2017-04-01
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Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00663/full |
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English |
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Article |
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DOAJ |
author |
Minsheng You Minsheng You Minsheng You Minsheng You Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Liette Vasseur Liette Vasseur Liette Vasseur Liette Vasseur Dandan Zheng Dandan Zheng Dandan Zheng Dandan Zheng Huanzi Zhong Bingcai Qin Junhan Lin Junhan Lin Junhan Lin Junhan Lin Yue Wang Yue Wang Yue Wang Yue Wang FengQin Song FengQin Song FengQin Song FengQin Song Yong Li Yong Li Yong Li Yong Li Hailan Lin Hailan Lin Hailan Lin Hailan Lin |
spellingShingle |
Minsheng You Minsheng You Minsheng You Minsheng You Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Liette Vasseur Liette Vasseur Liette Vasseur Liette Vasseur Dandan Zheng Dandan Zheng Dandan Zheng Dandan Zheng Huanzi Zhong Bingcai Qin Junhan Lin Junhan Lin Junhan Lin Junhan Lin Yue Wang Yue Wang Yue Wang Yue Wang FengQin Song FengQin Song FengQin Song FengQin Song Yong Li Yong Li Yong Li Yong Li Hailan Lin Hailan Lin Hailan Lin Hailan Lin Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory Frontiers in Microbiology diamondback moth bacteria diversity gut symbiosis carbohydrate biodegradation plant defense phenolic compounds |
author_facet |
Minsheng You Minsheng You Minsheng You Minsheng You Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Xiaofeng Xia Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Geoff M. Gurr Liette Vasseur Liette Vasseur Liette Vasseur Liette Vasseur Dandan Zheng Dandan Zheng Dandan Zheng Dandan Zheng Huanzi Zhong Bingcai Qin Junhan Lin Junhan Lin Junhan Lin Junhan Lin Yue Wang Yue Wang Yue Wang Yue Wang FengQin Song FengQin Song FengQin Song FengQin Song Yong Li Yong Li Yong Li Yong Li Hailan Lin Hailan Lin Hailan Lin Hailan Lin |
author_sort |
Minsheng You |
title |
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory |
title_short |
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory |
title_full |
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory |
title_fullStr |
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory |
title_full_unstemmed |
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory |
title_sort |
metagenomic sequencing of diamondback moth gut microbiome unveils key holobiont adaptations for herbivory |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-04-01 |
description |
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P. xylostella gut microbiota obtained by metagenomic sequencing. Gene enrichment in the metagenome, accompanied by functional identification, revealed an important role of specific gut bacteria in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids. Microbes participating in these pathways mainly belonged to three highly abundant bacteria: Enterobacter cloacae, Enterobacter asburiae, and Carnobacterium maltaromaticum. Results show that while the gut microbial community may be complex, a small number of functionally active species can be disproportionally important. The presence of specific enzymes in the microbiota community, such as supporting amino acid synthesis, digestion and detoxification functions, demonstrates the beneficial interactions between P. xylostella and its gut microbiota. These interactions can be potential targets for manipulation to provide novel pest management approaches. |
topic |
diamondback moth bacteria diversity gut symbiosis carbohydrate biodegradation plant defense phenolic compounds |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00663/full |
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doaj-ca862d84f7af44a3963c57be72da352a2020-11-24T21:44:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00663259102Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for HerbivoryMinsheng You0Minsheng You1Minsheng You2Minsheng You3Xiaofeng Xia4Xiaofeng Xia5Xiaofeng Xia6Xiaofeng Xia7Geoff M. Gurr8Geoff M. Gurr9Geoff M. Gurr10Geoff M. Gurr11Liette Vasseur12Liette Vasseur13Liette Vasseur14Liette Vasseur15Dandan Zheng16Dandan Zheng17Dandan Zheng18Dandan Zheng19Huanzi Zhong20Bingcai Qin21Junhan Lin22Junhan Lin23Junhan Lin24Junhan Lin25Yue Wang26Yue Wang27Yue Wang28Yue Wang29FengQin Song30FengQin Song31FengQin Song32FengQin Song33Yong Li34Yong Li35Yong Li36Yong Li37Hailan Lin38Hailan Lin39Hailan Lin40Hailan Lin41State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaGraham Centre, Charles Sturt UniversityOrange, NSW, AustraliaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaDepartment of Biological Sciences, Brock UniversitySt. Catharines, ON, CanadaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaBGI-ShenzhenShenzhen, ChinaBGI-ShenzhenShenzhen, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry UniversityFuzhou, ChinaInstitute of Applied Ecology, Fujian Agriculture and Forestry UniversityFuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of AgricultureFuzhou, ChinaFujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, ChinaHerbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P. xylostella gut microbiota obtained by metagenomic sequencing. Gene enrichment in the metagenome, accompanied by functional identification, revealed an important role of specific gut bacteria in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids. Microbes participating in these pathways mainly belonged to three highly abundant bacteria: Enterobacter cloacae, Enterobacter asburiae, and Carnobacterium maltaromaticum. Results show that while the gut microbial community may be complex, a small number of functionally active species can be disproportionally important. The presence of specific enzymes in the microbiota community, such as supporting amino acid synthesis, digestion and detoxification functions, demonstrates the beneficial interactions between P. xylostella and its gut microbiota. These interactions can be potential targets for manipulation to provide novel pest management approaches.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00663/fulldiamondback mothbacteria diversitygut symbiosiscarbohydrate biodegradationplant defensephenolic compounds |