The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a f...

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Main Authors: Pernille Badsberg Norup, Dorte L. Egeberg Palme, Morten R. Petersen, Katharina M. Main, Kristian Almstrup
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Journal of Clinical Medicine
Subjects:
Online Access:https://www.mdpi.com/2077-0383/9/8/2327
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spelling doaj-cac9dc23afcd405e93c7360831c8d1092020-11-25T03:02:22ZengMDPI AGJournal of Clinical Medicine2077-03832020-07-0192327232710.3390/jcm9082327The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with FecundityPernille Badsberg Norup0Dorte L. Egeberg Palme1Morten R. Petersen2Katharina M. Main3Kristian Almstrup4Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkEuropean Sperm Bank ApS, DK-2200 Copenhagen, DenmarkThe Fertility Clinic, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkDepartment of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, DenmarkThe acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) (<i>n</i> = 99) and intracytoplasmic sperm injection (ICSI) (<i>n</i> = 107) treatment. Purified frozen-thawed donor samples were also analyzed (<i>n</i> = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors (<i>p</i> = 5.3 × 10<sup>−8</sup>) and men from IVF couples (<i>p</i> = 2.2 × 10<sup>−5</sup>) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility (<i>p</i> = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, <i>p</i> = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.https://www.mdpi.com/2077-0383/9/8/2327fertility treatmentacrosomesperminfertilitymale factorsemen analysis
collection DOAJ
language English
format Article
sources DOAJ
author Pernille Badsberg Norup
Dorte L. Egeberg Palme
Morten R. Petersen
Katharina M. Main
Kristian Almstrup
spellingShingle Pernille Badsberg Norup
Dorte L. Egeberg Palme
Morten R. Petersen
Katharina M. Main
Kristian Almstrup
The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
Journal of Clinical Medicine
fertility treatment
acrosome
sperm
infertility
male factor
semen analysis
author_facet Pernille Badsberg Norup
Dorte L. Egeberg Palme
Morten R. Petersen
Katharina M. Main
Kristian Almstrup
author_sort Pernille Badsberg Norup
title The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
title_short The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
title_full The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
title_fullStr The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
title_full_unstemmed The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity
title_sort acrosomal status of density purified spermatozoa differentiates men from couples in ivf and icsi treatment and is associated with fecundity
publisher MDPI AG
series Journal of Clinical Medicine
issn 2077-0383
publishDate 2020-07-01
description The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) (<i>n</i> = 99) and intracytoplasmic sperm injection (ICSI) (<i>n</i> = 107) treatment. Purified frozen-thawed donor samples were also analyzed (<i>n</i> = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors (<i>p</i> = 5.3 × 10<sup>−8</sup>) and men from IVF couples (<i>p</i> = 2.2 × 10<sup>−5</sup>) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility (<i>p</i> = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, <i>p</i> = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.
topic fertility treatment
acrosome
sperm
infertility
male factor
semen analysis
url https://www.mdpi.com/2077-0383/9/8/2327
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