Mass Cultivation of Entomopathogenic Nematode in Artificial Media

• Main Authors: Dyah Rini Indriyanti, Nur Lailatul Muharromah
• Format: Article
• Language: English
• Published: Universitas Negeri Semarang 2016-03-01
• Series: Biosaintifika: Journal of Biology & Biology Education
• Subjects: cultivation media; Entomopathogenic Nematode; in-vitro
• Online Access: https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5579

<p>Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder), media E (yolk + chicken liver), media F (yolk + dog food), and media G (yeast + yolk + dog food). Each media was inoculated in 1.2x103JI/mL. The growth of EPN was observed weekly in 4 weeks. Results showed that EPN could be cultivated using various media; media D, E, F, and G. Highest abundance of EPN is found in the second week of media D for 28164 JI/ml.Cell harvesting is suggested to be conducted during the first and second week to obtain maximum abundance of EPN.</p><p><strong>How to Cite</strong></p><p>Indriyanti, D., &amp; Muharromah, N. (2016). Mass Cultivation of Entomopathogenic Nematode In Artificial Media.<em> Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(1), 111-118.</p>