sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9

sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9

The SNP within intron 3 of the porcine IGF2 gene (G3072A) plays an important role for muscle growth and fat deposition in pigs. In this study, the StCas9 derived from Streptococcus thermophilus together with the Drosha-mediated sgRNA-shRNA structure were combined to boost the G to A base editing on...

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Bibliographic Details
Main Authors: Yongsen Sun, Nana Yan, Lu Mu, Bing Sun, Jingrong Deng, Yuanyuan Fang, Simin Shao, Qiang Yan, Furong Han, Zhiying Zhang, Kun Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Genetics
Subjects:
IGF2 gene
SNP
base editing
CRISPR
StCas9
sgRNA-shRNA
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00347/full

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