Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates
Background: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytoki...
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Tehran University of Medical Sciences
2004-09-01
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doaj-caff1ce440bb4f3689441cb3030c3dfa2020-11-24T22:31:32ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222004-09-01624302309Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet ConcentratesShayegan MPoor Fath A ANomeiri MBabaei G RTarabadi F ABackground: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytokines level in PCs produced in Tehran Blood Center. Materials and Methods: This study proposed to determine if WBC reduction in PCs by pre-storage leuckodepletion filters reduced the levels of these cytokines during storage uo 3 days. Each of the PCs (n = 54) was prepared from single random donor (RD) by Platelet-Rich-Plasma (PRP), then were divided in four groups: 1- unfiltered non-irradiated RD-PCs (n= 13) 2- unfiltered and -irradiated RD-PCs (n=16) 3 - filtered non-irradiated RD-PCs (n=14) 4-filtered and -irradiated RD-PCs (n = 11). Cytokines levels in PCs supernatants were measured by ELISA kits according manufacture‘s instructions. Results: Our results showed : IL-8 was increased in unfiltered non-irradiated and IL-18 in -irradiated RD-PCs but not in the filtered RD-PCs in day 3 . Compared to unfiltered PCs in filtered units, pre-storage filtration prevented a rise in the IL-8 and TNF-  in day 3 of storage .The concentration of IL-1 was lower than the minimal detectable concentration by the kits used for this purpose. IL-6 was detected only in 7 units of all filtered PCs in day 3. Conclusion: These data indicate that pre-storage leuko-reduction of PCs can prevent accumulation of IL-6, IL-8 and TNF-  during storage. http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/969.pdf&manuscript_id=969FNHTRIL-6IL-8TNF- |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Shayegan M Poor Fath A A Nomeiri M Babaei G R Tarabadi F A |
spellingShingle |
Shayegan M Poor Fath A A Nomeiri M Babaei G R Tarabadi F A Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates Tehran University Medical Journal FNHTR IL-6 IL-8 TNF- |
author_facet |
Shayegan M Poor Fath A A Nomeiri M Babaei G R Tarabadi F A |
author_sort |
Shayegan M |
title |
Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates |
title_short |
Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates |
title_full |
Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates |
title_fullStr |
Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates |
title_full_unstemmed |
Level Of Cytokines (Like IL-1 , IL-6 , IL-8 And TNF- ) In Platelet Concentrates |
title_sort |
level of cytokines (like il-1 , il-6 , il-8 and tnf- ) in platelet concentrates |
publisher |
Tehran University of Medical Sciences |
series |
Tehran University Medical Journal |
issn |
1683-1764 1735-7322 |
publishDate |
2004-09-01 |
description |
Background: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytokines level in PCs produced in Tehran Blood Center. Materials and Methods: This study proposed to determine if WBC reduction in PCs by pre-storage leuckodepletion filters reduced the levels of these cytokines during storage uo 3 days. Each of the PCs (n = 54) was prepared from single random donor (RD) by Platelet-Rich-Plasma (PRP), then were divided in four groups: 1- unfiltered non-irradiated RD-PCs (n= 13) 2- unfiltered and -irradiated RD-PCs (n=16) 3 - filtered non-irradiated RD-PCs (n=14) 4-filtered and -irradiated RD-PCs (n = 11). Cytokines levels in PCs supernatants were measured by ELISA kits according manufacture‘s instructions. Results: Our results showed : IL-8 was increased in unfiltered non-irradiated and IL-18 in -irradiated RD-PCs but not in the filtered RD-PCs in day 3 . Compared to unfiltered PCs in filtered units, pre-storage filtration prevented a rise in the IL-8 and TNF-  in day 3 of storage .The concentration of IL-1 was lower than the minimal detectable concentration by the kits used for this purpose. IL-6 was detected only in 7 units of all filtered PCs in day 3. Conclusion: These data indicate that pre-storage leuko-reduction of PCs can prevent accumulation of IL-6, IL-8 and TNF-  during storage. |
topic |
FNHTR IL-6 IL-8 TNF- |
url |
http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/969.pdf&manuscript_id=969 |
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