Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry
Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems. Objective. To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS). Methods. Male Sp...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2013-01-01
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Series: | Disease Markers |
Online Access: | http://dx.doi.org/10.1155/2013/792391 |
Summary: | Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.
Objective. To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS). Methods. Male Sprague-Dawley rats () were randomly divided into 4 groups (/group): sham operation, day 1, day 3, and day 5. Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver. Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed. GC-MS was used to detect urinary metabolomics.
Results. Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated () and then decreased and reached close to normal levels at day 5. GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination. Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (). Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group. Conclusions. Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury. |
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ISSN: | 0278-0240 1875-8630 |