The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
Fertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecule...
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doaj-cb18e545004941ed8aca01773f457c5b2020-11-25T02:23:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01212651265110.3390/ijms21072651The Role of Taste Receptor mTAS1R3 in Chemical Communication of GametesMichaela Frolikova0Tereza Otcenaskova1Eliska Valasková2Pavla Postlerova3Romana Stopkova4Pavel Stopka5Katerina Komrskova6Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicFertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecules triggering various signaling pathways. This work focuses on a mouse taste receptor, mTAS1R3, encoded by the <i>Tas1r3</i> gene, as a potential receptor mediating chemical communication between gametes using the C57BL/6J lab mouse strain. In order to specify the role of mTAS1R3, we aimed to characterize its precise localization in testis and sperm using super resolution microscopy. The testis cryo-section, acrosome-intact sperm released from <i>cauda epididymis</i> and sperm which underwent the acrosome reaction (AR) were evaluated. The mTAS1R3 receptor was detected in late spermatids where the acrosome was being formed and in the acrosomal cap of acrosome intact sperm. AR is triggered in mice during sperm maturation in the female reproductive tract and by passing through the egg surroundings such as <i>cumulus oophorus</i> cells. This AR onset is independent of the extracellular matrix of the oocyte called <i>zona pellucida</i>. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid <i>L-glutamate</i>, naturally occurs. Therefore, we targeted the possible interaction in vitro between the mTAS1R3 and <i>L-glutamate</i> as a part of chemical communication between sperm and egg and used an anti-mTAS1R3-specific antibody to block it. We detected that the acrosome reacted spermatozoa showed a chemotactic response in the presence of <i>L-glutamate</i> during and after the AR, and it is likely that mTAS1R3 acted as its mediator.https://www.mdpi.com/1422-0067/21/7/2651acrosome reactionchemotaxischemoattractantgamete<i>L-glutamate</i>mouse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michaela Frolikova Tereza Otcenaskova Eliska Valasková Pavla Postlerova Romana Stopkova Pavel Stopka Katerina Komrskova |
spellingShingle |
Michaela Frolikova Tereza Otcenaskova Eliska Valasková Pavla Postlerova Romana Stopkova Pavel Stopka Katerina Komrskova The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes International Journal of Molecular Sciences acrosome reaction chemotaxis chemoattractant gamete <i>L-glutamate</i> mouse |
author_facet |
Michaela Frolikova Tereza Otcenaskova Eliska Valasková Pavla Postlerova Romana Stopkova Pavel Stopka Katerina Komrskova |
author_sort |
Michaela Frolikova |
title |
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes |
title_short |
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes |
title_full |
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes |
title_fullStr |
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes |
title_full_unstemmed |
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes |
title_sort |
role of taste receptor mtas1r3 in chemical communication of gametes |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-04-01 |
description |
Fertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecules triggering various signaling pathways. This work focuses on a mouse taste receptor, mTAS1R3, encoded by the <i>Tas1r3</i> gene, as a potential receptor mediating chemical communication between gametes using the C57BL/6J lab mouse strain. In order to specify the role of mTAS1R3, we aimed to characterize its precise localization in testis and sperm using super resolution microscopy. The testis cryo-section, acrosome-intact sperm released from <i>cauda epididymis</i> and sperm which underwent the acrosome reaction (AR) were evaluated. The mTAS1R3 receptor was detected in late spermatids where the acrosome was being formed and in the acrosomal cap of acrosome intact sperm. AR is triggered in mice during sperm maturation in the female reproductive tract and by passing through the egg surroundings such as <i>cumulus oophorus</i> cells. This AR onset is independent of the extracellular matrix of the oocyte called <i>zona pellucida</i>. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid <i>L-glutamate</i>, naturally occurs. Therefore, we targeted the possible interaction in vitro between the mTAS1R3 and <i>L-glutamate</i> as a part of chemical communication between sperm and egg and used an anti-mTAS1R3-specific antibody to block it. We detected that the acrosome reacted spermatozoa showed a chemotactic response in the presence of <i>L-glutamate</i> during and after the AR, and it is likely that mTAS1R3 acted as its mediator. |
topic |
acrosome reaction chemotaxis chemoattractant gamete <i>L-glutamate</i> mouse |
url |
https://www.mdpi.com/1422-0067/21/7/2651 |
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