The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes

Fertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecule...

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Main Authors: Michaela Frolikova, Tereza Otcenaskova, Eliska Valasková, Pavla Postlerova, Romana Stopkova, Pavel Stopka, Katerina Komrskova
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/21/7/2651
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spelling doaj-cb18e545004941ed8aca01773f457c5b2020-11-25T02:23:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01212651265110.3390/ijms21072651The Role of Taste Receptor mTAS1R3 in Chemical Communication of GametesMichaela Frolikova0Tereza Otcenaskova1Eliska Valasková2Pavla Postlerova3Romana Stopkova4Pavel Stopka5Katerina Komrskova6Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech RepublicDepartment of Zoology, Faculty of Science, Charles University, BIOCEV, Vinicna 7, 128 44 Prague 2, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech RepublicFertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecules triggering various signaling pathways. This work focuses on a mouse taste receptor, mTAS1R3, encoded by the <i>Tas1r3</i> gene, as a potential receptor mediating chemical communication between gametes using the C57BL/6J lab mouse strain. In order to specify the role of mTAS1R3, we aimed to characterize its precise localization in testis and sperm using super resolution microscopy. The testis cryo-section, acrosome-intact sperm released from <i>cauda epididymis</i> and sperm which underwent the acrosome reaction (AR) were evaluated. The mTAS1R3 receptor was detected in late spermatids where the acrosome was being formed and in the acrosomal cap of acrosome intact sperm. AR is triggered in mice during sperm maturation in the female reproductive tract and by passing through the egg surroundings such as <i>cumulus oophorus</i> cells. This AR onset is independent of the extracellular matrix of the oocyte called <i>zona pellucida</i>. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid <i>L-glutamate</i>, naturally occurs. Therefore, we targeted the possible interaction in vitro between the mTAS1R3 and <i>L-glutamate</i> as a part of chemical communication between sperm and egg and used an anti-mTAS1R3-specific antibody to block it. We detected that the acrosome reacted spermatozoa showed a chemotactic response in the presence of <i>L-glutamate</i> during and after the AR, and it is likely that mTAS1R3 acted as its mediator.https://www.mdpi.com/1422-0067/21/7/2651acrosome reactionchemotaxischemoattractantgamete<i>L-glutamate</i>mouse
collection DOAJ
language English
format Article
sources DOAJ
author Michaela Frolikova
Tereza Otcenaskova
Eliska Valasková
Pavla Postlerova
Romana Stopkova
Pavel Stopka
Katerina Komrskova
spellingShingle Michaela Frolikova
Tereza Otcenaskova
Eliska Valasková
Pavla Postlerova
Romana Stopkova
Pavel Stopka
Katerina Komrskova
The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
International Journal of Molecular Sciences
acrosome reaction
chemotaxis
chemoattractant
gamete
<i>L-glutamate</i>
mouse
author_facet Michaela Frolikova
Tereza Otcenaskova
Eliska Valasková
Pavla Postlerova
Romana Stopkova
Pavel Stopka
Katerina Komrskova
author_sort Michaela Frolikova
title The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
title_short The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
title_full The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
title_fullStr The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
title_full_unstemmed The Role of Taste Receptor mTAS1R3 in Chemical Communication of Gametes
title_sort role of taste receptor mtas1r3 in chemical communication of gametes
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2020-04-01
description Fertilization is a multiple step process leading to the fusion of female and male gametes and the formation of a zygote. Besides direct gamete membrane interaction via binding receptors localized on both oocyte and sperm surface, fertilization also involves gamete communication via chemical molecules triggering various signaling pathways. This work focuses on a mouse taste receptor, mTAS1R3, encoded by the <i>Tas1r3</i> gene, as a potential receptor mediating chemical communication between gametes using the C57BL/6J lab mouse strain. In order to specify the role of mTAS1R3, we aimed to characterize its precise localization in testis and sperm using super resolution microscopy. The testis cryo-section, acrosome-intact sperm released from <i>cauda epididymis</i> and sperm which underwent the acrosome reaction (AR) were evaluated. The mTAS1R3 receptor was detected in late spermatids where the acrosome was being formed and in the acrosomal cap of acrosome intact sperm. AR is triggered in mice during sperm maturation in the female reproductive tract and by passing through the egg surroundings such as <i>cumulus oophorus</i> cells. This AR onset is independent of the extracellular matrix of the oocyte called <i>zona pellucida</i>. After AR, the relocation of mTAS1R3 to the equatorial segment was observed and the receptor remained exposed to the outer surroundings of the female reproductive tract, where its physiological ligand, the amino acid <i>L-glutamate</i>, naturally occurs. Therefore, we targeted the possible interaction in vitro between the mTAS1R3 and <i>L-glutamate</i> as a part of chemical communication between sperm and egg and used an anti-mTAS1R3-specific antibody to block it. We detected that the acrosome reacted spermatozoa showed a chemotactic response in the presence of <i>L-glutamate</i> during and after the AR, and it is likely that mTAS1R3 acted as its mediator.
topic acrosome reaction
chemotaxis
chemoattractant
gamete
<i>L-glutamate</i>
mouse
url https://www.mdpi.com/1422-0067/21/7/2651
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