Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves

The flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and characterized. Direct and sequential soxhlet extraction and its concentrated fractions were subjected to thin layer chromatography and high performance thin layer chromatography. The results showed that maximum yi...

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Main Authors: Veena Sharma, Pracheta Janmeda
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Arabian Journal of Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1878535214001816
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spelling doaj-cb2690fbb8864120908e841169becbf82020-11-24T23:38:40ZengElsevierArabian Journal of Chemistry1878-53522017-05-0110450951410.1016/j.arabjc.2014.08.019Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leavesVeena SharmaPracheta JanmedaThe flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and characterized. Direct and sequential soxhlet extraction and its concentrated fractions were subjected to thin layer chromatography and high performance thin layer chromatography. The results showed that maximum yield of the flavonoid (6.53 g) was obtained from ethanolic extract. The Rf value of isolated flavonoid and phytochemical screening has been compared with standard Quercetin. Characterization of isolated flavonoid was done by IR, 1H NMR, and MS. On the basis of chemical and spectral analysis structure was elucidated as 2-(3,4-dihydroxy-5-methoxy-phenyl)-3,5-dihydroxy-6,7-dimethoxychromen-4-one, a flavonoid. This compound was isolated for the first time from this plant.http://www.sciencedirect.com/science/article/pii/S1878535214001816Euphorbia neriifoliaFlavonoidsChromatography
collection DOAJ
language English
format Article
sources DOAJ
author Veena Sharma
Pracheta Janmeda
spellingShingle Veena Sharma
Pracheta Janmeda
Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
Arabian Journal of Chemistry
Euphorbia neriifolia
Flavonoids
Chromatography
author_facet Veena Sharma
Pracheta Janmeda
author_sort Veena Sharma
title Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
title_short Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
title_full Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
title_fullStr Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
title_full_unstemmed Extraction, isolation and identification of flavonoid from Euphorbia neriifolia leaves
title_sort extraction, isolation and identification of flavonoid from euphorbia neriifolia leaves
publisher Elsevier
series Arabian Journal of Chemistry
issn 1878-5352
publishDate 2017-05-01
description The flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and characterized. Direct and sequential soxhlet extraction and its concentrated fractions were subjected to thin layer chromatography and high performance thin layer chromatography. The results showed that maximum yield of the flavonoid (6.53 g) was obtained from ethanolic extract. The Rf value of isolated flavonoid and phytochemical screening has been compared with standard Quercetin. Characterization of isolated flavonoid was done by IR, 1H NMR, and MS. On the basis of chemical and spectral analysis structure was elucidated as 2-(3,4-dihydroxy-5-methoxy-phenyl)-3,5-dihydroxy-6,7-dimethoxychromen-4-one, a flavonoid. This compound was isolated for the first time from this plant.
topic Euphorbia neriifolia
Flavonoids
Chromatography
url http://www.sciencedirect.com/science/article/pii/S1878535214001816
work_keys_str_mv AT veenasharma extractionisolationandidentificationofflavonoidfromeuphorbianeriifolialeaves
AT prachetajanmeda extractionisolationandidentificationofflavonoidfromeuphorbianeriifolialeaves
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