Optimization of N-hydroxysuccinimide ester coupling with aminoallyl-modified RNA for fluorescent labeling
Site-specific fluorescent labeling of RNA is crucial for obtaining the structural and dynamic information of RNAs by fluorescence techniques. Post-synthetic modification of RNA based on N-hydroxysuccinimide (NHS) coupling reaction is an economic, efficient and simple strategy to introduce fluorophor...
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2020-01-01
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Series: | Bioengineered |
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Online Access: | http://dx.doi.org/10.1080/21655979.2020.1765487 |