Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina
An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly i...
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doaj-cb6f95198caa4b61838500dac621e7ec2021-05-05T21:34:58ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.58635Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retinaAfnan Azizi0https://orcid.org/0000-0002-3288-9612Anne Herrmann1https://orcid.org/0000-0003-1745-7499Yinan Wan2Salvador JRP Buse3Philipp J Keller4https://orcid.org/0000-0003-2896-4920Raymond E Goldstein5https://orcid.org/0000-0003-2645-0598William A Harris6https://orcid.org/0000-0002-9995-8096Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United KingdomDepartment of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Cambridge, United KingdomHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, United StatesDepartment of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United KingdomHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, United StatesDepartment of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Cambridge, United KingdomDepartment of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United KingdomAn important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant.https://elifesciences.org/articles/58635nuclear crowdingdiffusioninterkinetic nuclear migration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Afnan Azizi Anne Herrmann Yinan Wan Salvador JRP Buse Philipp J Keller Raymond E Goldstein William A Harris |
spellingShingle |
Afnan Azizi Anne Herrmann Yinan Wan Salvador JRP Buse Philipp J Keller Raymond E Goldstein William A Harris Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina eLife nuclear crowding diffusion interkinetic nuclear migration |
author_facet |
Afnan Azizi Anne Herrmann Yinan Wan Salvador JRP Buse Philipp J Keller Raymond E Goldstein William A Harris |
author_sort |
Afnan Azizi |
title |
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_short |
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_full |
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_fullStr |
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_full_unstemmed |
Nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
title_sort |
nuclear crowding and nonlinear diffusion during interkinetic nuclear migration in the zebrafish retina |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-10-01 |
description |
An important question in early neural development is the origin of stochastic nuclear movement between apical and basal surfaces of neuroepithelia during interkinetic nuclear migration. Tracking of nuclear subpopulations has shown evidence of diffusion - mean squared displacements growing linearly in time - and suggested crowding from cell division at the apical surface drives basalward motion. Yet, this hypothesis has not yet been tested, and the forces involved not quantified. We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to track entire populations of nuclei within the tissue. The time-varying concentration profiles show clear evidence of crowding as nuclei reach close-packing and are quantitatively described by a nonlinear diffusion model. Considerations of nuclear motion constrained inside the enveloping cell membrane show that concentration-dependent stochastic forces inside cells, compatible in magnitude to those found in cytoskeletal transport, can explain the observed magnitude of the diffusion constant. |
topic |
nuclear crowding diffusion interkinetic nuclear migration |
url |
https://elifesciences.org/articles/58635 |
work_keys_str_mv |
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