Development of Novel Method for Immobilizing TMAH-Degrading Microbe into Pellet and Characterization Tool, for Verifying Its Robustness in Electronics Wastewater Treatment

• Main Authors: Seungjoon Chung, Jeongyun Choi, Jinwook Chung
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: International Journal of Environmental Research and Public Health
• Subjects: differential scanning calorimetry (DSC); electronics wastewater; immobilized cells; polymerization; tetramethyl ammonium hydroxide (TMAH)
• Online Access: https://www.mdpi.com/1660-4601/17/12/4411

This study describes an immobilization method of enriched microorganism, for robustly degrading organic compounds, including tetramethyl ammonium hydroxide (TMAH) in electronics wastewater without an increase of total organic carbon (TOC) in effluent. The enriched TMAH degrading bacteria was entrapped inside the pellets through polymerization. Polymerization conditions were optimized in terms of long-term TOC leak tests of pellet. Among several methods, a differential scanning calorimetry (DSC) analysis was found to be effective for the hands-on evaluation of stability in pellet. Stable pellets showed less than 10 J/g of curing heat by DSC analysis. This method is suitable for the optimization of polymerization conditions and controlling the quality of pellets. The removal efficiency of TMAH was over 95% and effluent concentration of TOC was below 100 ppb. The viability test results revealed that entrapped microorganisms were actively survived after five months of operations. This immobilization method is strongly suggested as a new strategy for the wastewater reuse process in low-strength electronics wastewater.