Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats
Hypericum perforatum L. (HP), a well-known natural medicine, has a potential effect on menopausal hypercholesterolemia. However, the effect of HP extract on gut microbiota and related metabolites, which play vital roles in metabolic disease occurrence, in the context of estrogen deficiency have not...
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Elsevier
2021-03-01
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Series: | Biomedicine & Pharmacotherapy |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S075333222031324X |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Chen Yanru Liu Zhishu Tang Xinbo Shi Zhongxing Song Fan Cao Peifeng Wei Min Li Xiaohong Li Dahai Jiang Yafeng Yan Ningjuan Yang |
spellingShingle |
Lin Chen Yanru Liu Zhishu Tang Xinbo Shi Zhongxing Song Fan Cao Peifeng Wei Min Li Xiaohong Li Dahai Jiang Yafeng Yan Ningjuan Yang Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats Biomedicine & Pharmacotherapy Gut microbiota Short-chain fatty acids Bile acids Hypericum perforatum L. Estrogen deficiency Hypercholesterolemia |
author_facet |
Lin Chen Yanru Liu Zhishu Tang Xinbo Shi Zhongxing Song Fan Cao Peifeng Wei Min Li Xiaohong Li Dahai Jiang Yafeng Yan Ningjuan Yang |
author_sort |
Lin Chen |
title |
Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats |
title_short |
Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats |
title_full |
Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats |
title_fullStr |
Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats |
title_full_unstemmed |
Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) rats |
title_sort |
improvements in estrogen deficiency-induced hypercholesterolemia by hypericum perforatum l. extract are associated with gut microbiota and related metabolites in ovariectomized (ovx) rats |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-03-01 |
description |
Hypericum perforatum L. (HP), a well-known natural medicine, has a potential effect on menopausal hypercholesterolemia. However, the effect of HP extract on gut microbiota and related metabolites, which play vital roles in metabolic disease occurrence, in the context of estrogen deficiency have not yet been reported. The aims of the present study were to investigate the effects of HP extract on gut microbial composition and related metabolite profiles in ovariectomized (OVX) rats and reveal the relationships between pathological indicators and alterations in both gut microbial composition at the genus level and metabolites. Body weight, serum parameters, liver lipids and histomorphology were determined. Microbial composition was analyzed using 16S rRNA sequencing. Fecal short-chain fatty acids (SCFAs) and serum bile acids were quantitatively measured. Correlations between pathological indicators and alteration in gut microbiota and metabolites were investigated using Spearman's rank correlation test. Gene expression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7α-hydroxylase (CYP7A1) and cholesterol 27-hydroxylase (CYP27A1) in the liver and G protein-coupled receptors (GPCRs; GPR43 and GPR41), ZO-1 and occludin in the cecum were determined by PCR. Microbial composition and metabolite profiles were significantly changed in OVX rats compared with sham rats. Twelve bacterial genera, 5 SCFAs and 12 bile acids were identified as differential biomarkers. Differential genera, SCFAs and bile acids were closely associated with weight, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C). In OVX rats, HP administration can significantly reverse the pathological symptoms of body weight gain, serum lipid disorders and hepatic steatosis, at the meanwhile, reestablish gut microbial composition and metabolite profiles. Moreover, HP administration significantly upregulated the levels of CYP7A1, GPR43 and GPR41. In conclusion, HP can ameliorate estrogen deficiency-induced hypercholesterolemia. The underlying mechanism may be associated with improvements in gut microbiota composition and the profile of related metabolites as well as increases in bile acid secretion. |
topic |
Gut microbiota Short-chain fatty acids Bile acids Hypericum perforatum L. Estrogen deficiency Hypercholesterolemia |
url |
http://www.sciencedirect.com/science/article/pii/S075333222031324X |
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doaj-cb8c6d5a45644992895963ca53d879092021-05-21T04:19:51ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-03-01135111131Improvements in estrogen deficiency-induced hypercholesterolemia by Hypericum perforatum L. extract are associated with gut microbiota and related metabolites in ovariectomized (OVX) ratsLin Chen0Yanru Liu1Zhishu Tang2Xinbo Shi3Zhongxing Song4Fan Cao5Peifeng Wei6Min Li7Xiaohong Li8Dahai Jiang9Yafeng Yan10Ningjuan Yang11Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR China; Corresponding authors.Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR China; Corresponding authors.Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaCollege of Pharmacy, Shaanxi University of Chinese Medicine, 712046, Xi’an, Shaanxi, PR ChinaCollege of Pharmacy, Shaanxi University of Chinese Medicine, 712046, Xi’an, Shaanxi, PR ChinaCollege of Pharmacy, Shaanxi University of Chinese Medicine, 712046, Xi’an, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaShaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine, 712083, Xianyang, Shaanxi, PR ChinaHypericum perforatum L. (HP), a well-known natural medicine, has a potential effect on menopausal hypercholesterolemia. However, the effect of HP extract on gut microbiota and related metabolites, which play vital roles in metabolic disease occurrence, in the context of estrogen deficiency have not yet been reported. The aims of the present study were to investigate the effects of HP extract on gut microbial composition and related metabolite profiles in ovariectomized (OVX) rats and reveal the relationships between pathological indicators and alterations in both gut microbial composition at the genus level and metabolites. Body weight, serum parameters, liver lipids and histomorphology were determined. Microbial composition was analyzed using 16S rRNA sequencing. Fecal short-chain fatty acids (SCFAs) and serum bile acids were quantitatively measured. Correlations between pathological indicators and alteration in gut microbiota and metabolites were investigated using Spearman's rank correlation test. Gene expression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, cholesterol 7α-hydroxylase (CYP7A1) and cholesterol 27-hydroxylase (CYP27A1) in the liver and G protein-coupled receptors (GPCRs; GPR43 and GPR41), ZO-1 and occludin in the cecum were determined by PCR. Microbial composition and metabolite profiles were significantly changed in OVX rats compared with sham rats. Twelve bacterial genera, 5 SCFAs and 12 bile acids were identified as differential biomarkers. Differential genera, SCFAs and bile acids were closely associated with weight, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C). In OVX rats, HP administration can significantly reverse the pathological symptoms of body weight gain, serum lipid disorders and hepatic steatosis, at the meanwhile, reestablish gut microbial composition and metabolite profiles. Moreover, HP administration significantly upregulated the levels of CYP7A1, GPR43 and GPR41. In conclusion, HP can ameliorate estrogen deficiency-induced hypercholesterolemia. The underlying mechanism may be associated with improvements in gut microbiota composition and the profile of related metabolites as well as increases in bile acid secretion.http://www.sciencedirect.com/science/article/pii/S075333222031324XGut microbiotaShort-chain fatty acidsBile acidsHypericum perforatum L.Estrogen deficiencyHypercholesterolemia |